Weiss Lab:TrafficPaper: Difference between revisions
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| [[Image:Figure1.png|thumb|left|Single-molecule dual-color total internal reflection fluorescence (TIRF) microscopy setups]] | | [[Image:Figure1.png|thumb|left|Single-molecule dual-color total internal reflection fluorescence (TIRF) microscopy setups]] | ||
| [[Image:Figure2.png|thumb|left|Crosslinking of Av-GPI induces patches in the absence of CTxB]] | | [[Image:Figure2.png|thumb|left|Crosslinking of Av-GPI induces patches in the absence of CTxB]] | ||
| Comparison between single-trajectory PDSD analysis and global PDSD analysis | | [[Image:Figure3.png|thumb|left|Comparison between single-trajectory PDSD analysis and global PDSD analysis | ||
| Distribution of Av-GPI diffusion coefficients in untreated HeLa cells at 37°C | | [[Image:Figure4.png|thumb|left|Distribution of Av-GPI diffusion coefficients in untreated HeLa cells at 37°C | ||
|- | |- | ||
| Visualization of slow/immobile molecules using mean intensity projection images | | [[Image:Figure5.png|thumb|left|Visualization of slow/immobile molecules using mean intensity projection images | ||
| Immunostaining of endogenous caveolin-1 and colocalization with caveolin-1-EGFP in two HeLa cell lines | | [[Image:Figure6.png|thumb|left|Immunostaining of endogenous caveolin-1 and colocalization with caveolin-1-EGFP in two HeLa cell lines | ||
| Quantification of Av-GPI membrane expression in Av-GPI and Av-GPI/Cav1-EGFP expressing HeLa cells | | [[Image:Figure7.png|thumb|left|Quantification of Av-GPI membrane expression in Av-GPI and Av-GPI/Cav1-EGFP expressing HeLa cells | ||
|Diffusion coefficients and confinement sizes of Av-GPI for various treatments of HeLa cells at room temperature | | [[Image:Figure8.png|thumb|left|Diffusion coefficients and confinement sizes of Av-GPI for various treatments of HeLa cells at room temperature | ||
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Revision as of 16:49, 16 February 2009
Additional data for
Dynamic Partitioning of a GPI-Anchored Protein in Glycosphingolipid-Rich Microdomains Imaged by Single-Quantum Dot Tracking
Fabien Pinaud[1,3], Xavier Michalet[1,3], Gopal Iyer[1], Emmanuel Margeat[1], Hsiao-Ping Moore[2], Shimon Weiss[1,3].
[1]Department of Chemistry & Biochemistry, University of California, Los Angeles, CA, USA.
[2]Department of Molecular & Cell Biology, University of California, Berkeley, CA, USA.
[3]Corresponding authors: fabien.pinaud@lkb.ens.fr; michalet@chem.ucla.edu and sweiss@chem.ucla.edu
Published in Traffic
Note: The following additional data are part of the supplementary information that were submitted and reviewed for publication of the manuscript in Traffic. Unfortunately, because of size constrains, these additional data could not be posted together with the rest of the supplementary information on Traffic website.
These data include:
[[Image:Figure3.png|thumb|left|Comparison between single-trajectory PDSD analysis and global PDSD analysis | [[Image:Figure4.png|thumb|left|Distribution of Av-GPI diffusion coefficients in untreated HeLa cells at 37°C | ||
[[Image:Figure5.png|thumb|left|Visualization of slow/immobile molecules using mean intensity projection images | [[Image:Figure6.png|thumb|left|Immunostaining of endogenous caveolin-1 and colocalization with caveolin-1-EGFP in two HeLa cell lines | [[Image:Figure7.png|thumb|left|Quantification of Av-GPI membrane expression in Av-GPI and Av-GPI/Cav1-EGFP expressing HeLa cells | [[Image:Figure8.png|thumb|left|Diffusion coefficients and confinement sizes of Av-GPI for various treatments of HeLa cells at room temperature |