Western Blots: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| Line 1: | Line 1: | ||
== SDS-PAGE Westerns == | == SDS-PAGE Westerns == | ||
| Line 9: | Line 8: | ||
===Procedure=== | ===Procedure=== | ||
====Prepare Samples==== | |||
*Boil Samples for 5 min, cool on ice 1 min, spin 1 min | *Boil Samples for 5 min, cool on ice 1 min, spin 1 min | ||
*Ladders: 8ul ladder + 8ul 2x PLS | |||
**High mol weight 30KDa - 220 KDa | |||
**Low mol weight 6.5KDa - 45 Kda | |||
Revision as of 09:05, 31 August 2006
SDS-PAGE Westerns
Materials
- 2x PLS
- PBS-T
- 5% milk in PBS-T
Procedure
Prepare Samples
- Boil Samples for 5 min, cool on ice 1 min, spin 1 min
- Ladders: 8ul ladder + 8ul 2x PLS
- High mol weight 30KDa - 220 KDa
- Low mol weight 6.5KDa - 45 Kda
