Western Blots

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(Procedure)
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== SDS-PAGE Westerns ==
== SDS-PAGE Westerns ==
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===Procedure===
===Procedure===
 +
====Prepare Samples====
*Boil Samples for 5 min, cool on ice 1 min, spin 1 min
*Boil Samples for 5 min, cool on ice 1 min, spin 1 min
 +
*Ladders: 8ul ladder + 8ul 2x PLS
 +
**High mol weight 30KDa - 220 KDa
 +
**Low mol weight 6.5KDa - 45 Kda

Revision as of 12:05, 31 August 2006

Contents

SDS-PAGE Westerns

Materials

  • 2x PLS
  • PBS-T
  • 5% milk in PBS-T

Procedure

Prepare Samples

  • Boil Samples for 5 min, cool on ice 1 min, spin 1 min
  • Ladders: 8ul ladder + 8ul 2x PLS
    • High mol weight 30KDa - 220 KDa
    • Low mol weight 6.5KDa - 45 Kda
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