Wikiomics:RNA-Seq: Difference between revisions

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current version: 2010-07-27
current version: 2010-07-27


FastA input only, support for paired ends.
FastA and FASTQ  input, support for paired ends.
 


===ERANGE===
===ERANGE===

Revision as of 18:15, 23 November 2010

Mappers

Spliced Mappers (tested)

Tophat

http://tophat.cbcb.umd.edu/

current version: 1.1.4 release 2010.11.16

base mapper: bowtie

SOAPals

http://soap.genomics.org.cn/soapals.html

current version: 1.1 , 05-05-2010


GEM

http://sourceforge.net/apps/mediawiki/gemlibrary/index.php?title=The_GEM_library

current version: GEM-binaries-Linux-x86_64-20100419-003425.tbz2 base mapper: GEM split-mapper

GMAP/GSNAP

http://research-pub.gene.com/gmap/

current version: 2010-07-27

FastA and FASTQ input, support for paired ends.

ERANGE

http://woldlab.caltech.edu/rnaseq/

current version: 3.2.1 from 2010.08.10

base mapper: bowtie or blat


SOLiD data only

(untested)

SplitSeek

http://solidsoftwaretools.com/gf/project/splitseek/

current version: 1.3.2

RNA-mate

http://solidsoftwaretools.com/gf/project/rnamate

current version: 1.01

Spliced Mappers (in developement)

Mapsplice

http://www.netlab.uky.edu/p/bioinfo/MapSplice/

current version: MapSplice 1.14.1 2010.09.30

base mapper: bowtie

SpliceMap

http://www.stanford.edu/group/wonglab/SpliceMap/

current version: 3.3.5.2 2010.10.23

base mapper (preferred): bowtie (others possible) "Currently, only the cannoical GT-AG splice sites are identified."

TAU

http://mocklerlab-tools.cgrb.oregonstate.edu/TAU.html

current version: 1.4 2010.09.06


HMMSplicer

http://derisilab.ucsf.edu/index.php?software=105

current version: 0.9.0 2010.08.30

Developed in Python.

SAW (method no software yet)

Ning K, Fermin D (2010) SAW: A Method to Identify Splicing Events from RNA-Seq Data Based on Splicing Fingerprints. PLoS ONE 5(8): e12047. doi:10.1371/journal.pone.0012047

Spliced Mappers (old)

GMORSE

http://www.genoscope.cns.fr/externe/gmorse/ Proper name: G-Mo.R-Se current version: 06-Nov-2009

It was used for Vitis vinifera genome project.

Not spliced

Mapping short reads to draft genome sequence with multiple contigs poses problems for current spliced mappers.

blat

http://genome.ucsc.edu/FAQ/FAQblat.html

last

http://last.cbrc.jp/

Latest: last-139.zip 13-Oct-2010

Installation:

cd src; make