Wikiomics:Site Directed Mutagenesis - Revision history

Edward Clements at 03:38, 6 March 2010

2010-03-06T03:38:15Z

←Older revision		Revision as of 03:38, 6 March 2010
Line 1:		Line 1:
-	This page is beta. ~~please~~ feel free to update it.	+	This page is beta. Please feel free to update it.

	The idea here is to create a flow of work to simulate the point mutation of a single amino acid in a protein.		The idea here is to create a flow of work to simulate the point mutation of a single amino acid in a protein.

Edward Clements at 03:37, 6 March 2010

2010-03-06T03:37:36Z

Torsten Waldminghaus: added tags

2008-03-03T21:24:35Z

added tags

←Older revision		Revision as of 21:24, 3 March 2008
Line 17:		Line 17:
	#If the protein is a multiple subunit protein - what are the subunit interaction residues ? (CSU)		#If the protein is a multiple subunit protein - what are the subunit interaction residues ? (CSU)

-	[[Category:~~Sequence~~]]	+	[[Category:Protocol]] [[Category:In silico]] [[Category:Cloning]]

Bill Flanagan at 05:29, 20 November 2007

2007-11-20T05:29:32Z

←Older revision		Revision as of 05:29, 20 November 2007
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	##if not check out [http://speedy.embl-heidelberg.de/gtsp/flowchart2.html STRUCTURE PREDICTION FLOWCHART]		##if not check out [http://speedy.embl-heidelberg.de/gtsp/flowchart2.html STRUCTURE PREDICTION FLOWCHART]
	#What are the known domains inside our protein ?		#What are the known domains inside our protein ?
-	## see [[Searching for 3D functional sites in a protein structure]]	+	## see [[Wikiomics:Searching for 3D functional sites in a protein structure]]
	## Where is the Active Site ? ([http://bioinfo2.weizmann.ac.il/~pietro/SARIG/V3/index.html SARIG])		## Where is the Active Site ? ([http://bioinfo2.weizmann.ac.il/~pietro/SARIG/V3/index.html SARIG])
	## What is the location of the mutation? (surface / inside the protein)		## What is the location of the mutation? (surface / inside the protein)

Bill Flanagan: 1 revision(s)

2007-11-20T04:01:06Z

1 revision(s)

←Older revision

Revision as of 04:01, 20 November 2007

194.214.217.17 at 14:09, 2 June 2006

2006-06-02T14:09:16Z

New page

This page is beta. please feel free to update it.

The idea here is to create a flow of work to simulate the point mutation of a single amino acid in a protein.

#First step is to choose your target. (This is up to you).
#Finding the nucleic sequence ([http://www.ncbi.nlm.nih.gov/entrez/query.fcgi ENTREZ])
#What type of mutation will be introduced ? (substitution / deletion / addition)
#Can we find the wanted mutation in other related proteins ? (Blast)
#How conserved is the protein we wish to mutate ?
#Does the w.t. protein has a known 3D structure ? (Check [http://www.rcsb.org/pdb/ PDB])
##if not check out [http://speedy.embl-heidelberg.de/gtsp/flowchart2.html STRUCTURE PREDICTION FLOWCHART]
#What are the known domains inside our protein ?
## see [[Searching for 3D functional sites in a protein structure]]
## Where is the Active Site ? ([http://bioinfo2.weizmann.ac.il/~pietro/SARIG/V3/index.html SARIG])
## What is the location of the mutation? (surface / inside the protein)
#What impact does the mutation have on the protein (no backbone changes yet)
#If the protein is a multiple subunit protein - what are the subunit interaction residues ? (CSU)

[[Category:Sequence]]

←Older revision		Revision as of 03:37, 6 March 2010
Line 3:		Line 3:
	The idea here is to create a flow of work to simulate the point mutation of a single amino acid in a protein.		The idea here is to create a flow of work to simulate the point mutation of a single amino acid in a protein.

-	#First step is to choose your target. (This is up to you).	+	#First step is to choose your target. (This is up to you.)
	#Finding the nucleic sequence ([http://www.ncbi.nlm.nih.gov/entrez/query.fcgi ENTREZ])		#Finding the nucleic sequence ([http://www.ncbi.nlm.nih.gov/entrez/query.fcgi ENTREZ])
	#What type of mutation will be introduced ? (substitution / deletion / addition)		#What type of mutation will be introduced ? (substitution / deletion / addition)
	#Can we find the wanted mutation in other related proteins ? (Blast)		#Can we find the wanted mutation in other related proteins ? (Blast)
	#How conserved is the protein we wish to mutate ?		#How conserved is the protein we wish to mutate ?
-	#Does the w.t. protein ~~has~~ a known 3D structure ? (Check [http://www.rcsb.org/pdb/ PDB])	+	#Does the w.t. protein have a known 3D structure ? (Check [http://www.rcsb.org/pdb/ PDB])
-	##if not check out [http://speedy.embl-heidelberg.de/gtsp/flowchart2.html STRUCTURE PREDICTION FLOWCHART]	+	##If not, check out [http://speedy.embl-heidelberg.de/gtsp/flowchart2.html STRUCTURE PREDICTION FLOWCHART]
	#What are the known domains inside our protein ?		#What are the known domains inside our protein ?
	## see [[Wikiomics:Searching for 3D functional sites in a protein structure]]		## see [[Wikiomics:Searching for 3D functional sites in a protein structure]]
	## Where is the Active Site ? ([http://bioinfo2.weizmann.ac.il/~pietro/SARIG/V3/index.html SARIG])		## Where is the Active Site ? ([http://bioinfo2.weizmann.ac.il/~pietro/SARIG/V3/index.html SARIG])
	## What is the location of the mutation? (surface / inside the protein)		## What is the location of the mutation? (surface / inside the protein)
-	#What impact does the mutation have on the protein (no backbone changes yet)	+	#What impact does the mutation have on the protein? (no backbone changes yet)
	#If the protein is a multiple subunit protein - what are the subunit interaction residues ? (CSU)		#If the protein is a multiple subunit protein - what are the subunit interaction residues ? (CSU)

	[[Category:Protocol]] [[Category:In silico]] [[Category:Cloning]]		[[Category:Protocol]] [[Category:In silico]] [[Category:Cloning]]