Will Deloache Test Page: Difference between revisions
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The toggle-point of the plasmids was first investigated to determine the amount of input that must be detected by the cell for a toggle to occur. This was looked at specifically for IPTG. Figure 2 shows that a step-like toggle occurs at an IPTG concentration between 10<sup>-4</sup> and 10<sup>-5</sup> M. Based on this data, 100% of cells should “remember” an IPTG concentration >10<sup>-4</sup> M, while a concentration below this will cause none or a portion of the cells to toggle. Similar measurements were done for aTc concentration and lambda temperature sensitivity. | The toggle-point of the plasmids was first investigated to determine the amount of input that must be detected by the cell for a toggle to occur. This was looked at specifically for IPTG. Figure 2 shows that a step-like toggle occurs at an IPTG concentration between 10<sup>-4</sup> and 10<sup>-5</sup> M. Based on this data, 100% of cells should “remember” an IPTG concentration >10<sup>-4</sup> M, while a concentration below this will cause none or a portion of the cells to toggle. Similar measurements were done for aTc concentration and lambda temperature sensitivity. | ||
[[Image:linebreak.png]] | [[Image:linebreak.png]] | ||
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The pTAK and pIKE constructs were then tested for the ability to toggle between the on and off states. In Figure 2, the top row of boxes a and b represent the experimental results for the different plasmid types. The bottom two rows of boxes a and b are controls, which behave as expected. All of the cells were started in the on state though 6 hours of exposure to IPTG. All but one of the experimental constructs remained in the on state after removal of IPTG. The promoter and repressor strength were likely not well-balanced in the construct that didn’t retain memory. The cells were then exposed to their second input (42 degrees C or aTc), causing them to toggle into the off state and remain off even when the input was removed. | The pTAK and pIKE constructs were then tested for the ability to toggle between the on and off states. In Figure 2, the top row of boxes a and b represent the experimental results for the different plasmid types. The bottom two rows of boxes a and b are controls, which behave as expected. All of the cells were started in the on state though 6 hours of exposure to IPTG. All but one of the experimental constructs remained in the on state after removal of IPTG. The promoter and repressor strength were likely not well-balanced in the construct that didn’t retain memory. The cells were then exposed to their second input (42 degrees C or aTc), causing them to toggle into the off state and remain off even when the input was removed. | ||
[[Image:ToggleResults.png|thumb| | [[Image:ToggleResults.png|thumb|400px|center|'''Figure 3: Experimental results for a) pTAK and b) pIKE plasmids.''']] | ||
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==Conclusions== | ==Conclusions== |
Revision as of 13:35, 3 December 2007
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