Wittrup:Buffers and media: Difference between revisions
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'''Complex Media''' | '''Complex Media''' | ||
*1000 mL YPD | *1000 mL YPD | ||
*10g Yeast extract | **10g Yeast extract | ||
*20g Peptone | **20g Peptone | ||
*20g Dextrose | **20g Dextrose | ||
Add ddH2O to 1 L. Autoclave or filter sterilize. The dextrose may carmelize in the autoclave, which shouldn't hurt the media, but will make it dark. For filter sterilization, it will be very thick, so use a vacu-cap Gelman filter. | Add ddH2O to 1 L. Autoclave or filter sterilize. The dextrose may carmelize in the autoclave, which shouldn't hurt the media, but will make it dark. For filter sterilization, it will be very thick, so use a vacu-cap Gelman filter. | ||
Revision as of 11:43, 10 February 2006
Buffers
- Wittrup: EDTA
- Wittrup: TE (pH 8.0)
- Wittrup: PBS (pH 7.4)
- Wittrup: HBS (pH 7.4)
- Wittrup: TAE (Tris-Acetate)
- Wittrup: TBE (Tris-Borate)
Yeast Media
Introduction
- YPD (complex): No selection, rich medium, very good cell density
- SD(G)-CAA (Minimal): Trp, Ura, Ade selection, average cell density
Complex Media
- 1000 mL YPD
- 10g Yeast extract
- 20g Peptone
- 20g Dextrose
Add ddH2O to 1 L. Autoclave or filter sterilize. The dextrose may carmelize in the autoclave, which shouldn't hurt the media, but will make it dark. For filter sterilization, it will be very thick, so use a vacu-cap Gelman filter.
Minimal Media
