Wittrup:Buffers and media: Difference between revisions
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*SD(G)-CAA (Minimal): Trp, Ura, Ade selection, average cell density | *SD(G)-CAA (Minimal): Trp, Ura, Ade selection, average cell density | ||
'''Complex Media''' | '''Complex Media''' | ||
*1000 mL | *YPD, 1000 mL | ||
**10g Yeast extract | **10g Yeast extract | ||
**20g Peptone | **20g Peptone | ||
**20g Dextrose | **20g Dextrose | ||
*Agar Plates, 1000 mL | |||
**15g Bacto-agar | |||
Add ddH2O to 1 L. Autoclave or filter sterilize. The dextrose may carmelize in the autoclave, which shouldn't hurt the media, but will make it dark. For filter sterilization, it will be very thick, so use a vacu-cap Gelman filter. | Add ddH2O to 1 L. Autoclave or filter sterilize. The dextrose may carmelize in the autoclave, which shouldn't hurt the media, but will make it dark. For filter sterilization, it will be very thick, so use a vacu-cap Gelman filter. | ||
'''Minimal Media''' | '''Minimal Media''' | ||
Revision as of 11:45, 10 February 2006
Buffers
- Wittrup: EDTA
- Wittrup: TE (pH 8.0)
- Wittrup: PBS (pH 7.4)
- Wittrup: HBS (pH 7.4)
- Wittrup: TAE (Tris-Acetate)
- Wittrup: TBE (Tris-Borate)
Yeast Media
Introduction
- YPD (complex): No selection, rich medium, very good cell density
- SD(G)-CAA (Minimal): Trp, Ura, Ade selection, average cell density
Complex Media
- YPD, 1000 mL
- 10g Yeast extract
- 20g Peptone
- 20g Dextrose
- Agar Plates, 1000 mL
- 15g Bacto-agar
Add ddH2O to 1 L. Autoclave or filter sterilize. The dextrose may carmelize in the autoclave, which shouldn't hurt the media, but will make it dark. For filter sterilization, it will be very thick, so use a vacu-cap Gelman filter.
Minimal Media
