Wittrup:Protocols

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(DNA)
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===DNA===
===DNA===
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[[Wittrup:  Plasmid minipreps, restriction digestion]]
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[[Wittrup:  Gel electrophoresis and band purification]]
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[[Wittrup:  Site directed mutagenesis by QuikChange]]
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[[Wittrup:  PCR]]
===RNA===
===RNA===

Revision as of 19:40, 15 January 2006

Contents

Buffers/Equipment/Media

Wittrup:Buffers

Wittrup:Yeast media

Assays

DNA

Wittrup: Plasmid minipreps, restriction digestion

Wittrup: Gel electrophoresis and band purification

Wittrup: Site directed mutagenesis by QuikChange

Wittrup: PCR

RNA

Proteins

Wittrup: Determining Protein Concentration

Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)

Wittrup:FPLC general use

Wittrup:SDS-PAGE

Wittrup:ECL Western Blots and Phosphorimager

Wittrup:Protein concentration by ultrafiltration


Yeast Protocols

Wittrup: Yeast Transformation

Wittrup: Plasmid rescue from yeast

Wittrup: Yeast storage and revival

Wittrup: Yeast surface display

Cell culture

Software

Wittrup: Sequence alignment

Wittrup: PCR primer design

Wittrup: Protein visualization (PyMOL)

Wittrup: Biology Workbench

Personal tools