Wittrup:Protocols: Difference between revisions
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===Recipes=== | ===Recipes=== | ||
[[Wittrup:Buffers | [[Wittrup: Buffers]] | ||
[[Wittrup: Yeast Media/Buffers]] | |||
[[Wittrup: E. coli Media]] | |||
===Equipment=== | ===Equipment=== | ||
Revision as of 12:12, 10 February 2006
Recipes
Equipment
Wittrup:Deltavision deconvolution microscope
DNA
Wittrup: Plasmid minipreps, restriction digestion
Wittrup: Gel electrophoresis and band purification
Wittrup: Site directed mutagenesis by QuikChange
Proteins
Wittrup: Determining Protein Concentration
Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)
Wittrup:ECL Western Blots and Phosphorimager
Wittrup:Protein concentration by ultrafiltration
Wittrup:Protein conjugation with fluorophores
Yeast Protocols
Wittrup: Plasmid rescue from yeast
Wittrup: Yeast storage and revival
Wittrup: Yeast surface display
Cell culture
Wittrup: Cell culture protocols
