Wittrup:Protocols
From OpenWetWare
(→Cell culture) |
(→Proteins) |
||
| Line 48: | Line 48: | ||
[[Wittrup: TCA Protein Precipitation]] | [[Wittrup: TCA Protein Precipitation]] | ||
| + | |||
| + | [[Wittrup: TEV Protease Purification and Digestion]] | ||
===Yeast Protocols=== | ===Yeast Protocols=== | ||
Revision as of 11:30, 15 June 2009
Contents |
Recipes
Equipment
Wittrup:Deltavision deconvolution microscope
DNA
Wittrup: Restriction digestion
Wittrup: Site directed mutagenesis by QuikChange
Proteins
Wittrup: Determining Protein Concentration
Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)
Wittrup: ECL Western Blots and Phosphorimager
Wittrup: Protein concentration by ultrafiltration
Wittrup: Protein conjugation with fluorophores
Wittrup: Fluorescein Quench Titration Assay
Wittrup: TCA Protein Precipitation
Wittrup: TEV Protease Purification and Digestion
Yeast Protocols
Wittrup: Plasmid rescue from yeast
Wittrup: Yeast storage and revival
Wittrup: Yeast surface display
Wittrup: Cell Surface Secretion Assay
Wittrup: Fluorescence Microscopy of Yeast
Wittrup: Yeast Preps for Western Blotting
Cell culture
Wittrup: Subculturing Protocol
