Wittrup:Protocols: Difference between revisions
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[[Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)]] | [[Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)]] | ||
[[Wittrup:FPLC general use]] | [[Wittrup: FPLC general use]] | ||
[[Wittrup:SDS-PAGE]] | [[Wittrup: SDS-PAGE]] | ||
[[Wittrup:ECL Western Blots and Phosphorimager]] | [[Wittrup: ECL Western Blots and Phosphorimager]] | ||
[[Wittrup:Protein concentration by ultrafiltration]] | [[Wittrup: Protein concentration by ultrafiltration]] | ||
[[Wittrup:Protein conjugation with fluorophores]] | [[Wittrup: Protein conjugation with fluorophores]] | ||
[[Wittrup: Fluorescein Quench Titration Assay]] | |||
[[Wittrup: TCA Protein Precipitation]] | |||
===Yeast Protocols=== | ===Yeast Protocols=== | ||
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[[Wittrup: Yeast surface display]] | [[Wittrup: Yeast surface display]] | ||
[[Wittrup: Cell Surface Secretion Assay]] | |||
[[Wittrup: Fluorescence Microscopy of Yeast]] | |||
===Cell culture=== | ===Cell culture=== | ||
Revision as of 11:26, 24 July 2006
Recipes
Equipment
Wittrup:Deltavision deconvolution microscope
DNA
Wittrup: Restriction digestion
Wittrup: Site directed mutagenesis by QuikChange
Proteins
Wittrup: Determining Protein Concentration
Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)
Wittrup: ECL Western Blots and Phosphorimager
Wittrup: Protein concentration by ultrafiltration
Wittrup: Protein conjugation with fluorophores
Wittrup: Fluorescein Quench Titration Assay
Wittrup: TCA Protein Precipitation
Yeast Protocols
Wittrup: Plasmid rescue from yeast
Wittrup: Yeast storage and revival
Wittrup: Yeast surface display
Wittrup: Cell Surface Secretion Assay
Wittrup: Fluorescence Microscopy of Yeast
Cell culture
Wittrup: Subculturing Protocol
Wittrup: Cell Counting Protocol
