Wittrup:Protocols

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Current revision (12:59, 16 August 2012) (view source)
(Animal studies)
 
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===Buffers/Equipment/Media===
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===General Lab Duties===
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[[Wittrup:Buffers]]
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[[Wittrup: Dishwashing]]
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[[Wittrup:Yeast media]]
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===Recipes===
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===Assays===
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[[Wittrup: Buffers]]
 +
[[Wittrup: Yeast Media/Buffers]]
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 +
[[Wittrup: E. coli Media]]
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===Equipment===
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[[Wittrup:XL Flow cytometer]]
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[[Wittrup:Deltavision deconvolution microscope]]
===DNA===
===DNA===
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===RNA===
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[[Wittrup:  Plasmid miniprep]]
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[[Wittrup:  Restriction digestion]]
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[[Wittrup:  Gel electrophoresis]]
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[[Wittrup:  Gel extraction]]
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[[Wittrup:  Site directed mutagenesis by QuikChange]]
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[[Wittrup:  PCR]]
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[[Wittrup:  Yeast colony PCR]]
===Proteins===
===Proteins===
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[[Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)]]
[[Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)]]
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[[Wittrup:FPLC general use]]
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[[Wittrup: FPLC general use]]
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[[Wittrup:SDS-PAGE]]
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[[Wittrup: SDS-PAGE]]
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[[Wittrup:ECL Western Blots and Phosphorimager]]
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[[Wittrup: ECL Western Blots and Phosphorimager]]
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[[Wittrup:Protein concentration by ultrafiltration]]
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[[Wittrup: Protein concentration by ultrafiltration]]
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[[Wittrup:  Protein conjugation with fluorophores]]
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[[Wittrup:  Fluorescein Quench Titration Assay]]
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[[Wittrup:  TCA Protein Precipitation]]
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[[Wittrup:  TEV Protease Purification and Digestion]]
===Yeast Protocols===
===Yeast Protocols===
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[[Wittrup:  Yeast surface display]]
[[Wittrup:  Yeast surface display]]
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[[Wittrup:  Cell Surface Secretion Assay]]
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[[Wittrup:  Fluorescence Microscopy of Yeast]]
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[[Wittrup:  Yeast Preps for Western Blotting]]
===Cell culture===
===Cell culture===
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===Software===
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[[Wittrup: New Cell Line Banking and Authentication]]
 +
 
 +
[[Wittrup: Basic Culture Media]]
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[[Wittrup: Recovery Media]]
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[[Wittrup: Freezing Media]]
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[[Wittrup: Freezing Protocol]]
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[[Wittrup: Subculturing Protocol]]
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[[Wittrup: Thawing Protocol]]
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[[Wittrup: Crystal Violet Toxicity Assay Protocol]]
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[[Wittrup: HEK293-F Transfection]]
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[[Wittrup: Mycoplasma Detection and Clearance]]
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===Animal studies===
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[[Wittrup:Immunohistochemistry of frozen sections]]
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[[Wittrup:Immunohistochemistry of paraffin embedded sections]]
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[[Wittrup:Immunofluorescence of frozen sections]]
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[[Wittrup: Sequence alignment]]
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[[Wittrup:Tumor dissociation]]
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[[Wittrup: PCR primer design]]
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[[Wittrup:Making a cell line from tumor sections]]
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[[Wittrup:  Protein visualization (PyMOL)]]
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===Individual Lab Member Protocols===
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[[Wittrup: Biology Workbench]]
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[[User: Cary F Opel]]

Current revision

Contents

General Lab Duties

Wittrup: Dishwashing

Recipes

Wittrup: Buffers

Wittrup: Yeast Media/Buffers

Wittrup: E. coli Media

Equipment

Wittrup:XL Flow cytometer

Wittrup:Deltavision deconvolution microscope

DNA

Wittrup: Plasmid miniprep

Wittrup: Restriction digestion

Wittrup: Gel electrophoresis

Wittrup: Gel extraction

Wittrup: Site directed mutagenesis by QuikChange

Wittrup: PCR

Wittrup: Yeast colony PCR

Proteins

Wittrup: Determining Protein Concentration

Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)

Wittrup: FPLC general use

Wittrup: SDS-PAGE

Wittrup: ECL Western Blots and Phosphorimager

Wittrup: Protein concentration by ultrafiltration

Wittrup: Protein conjugation with fluorophores

Wittrup: Fluorescein Quench Titration Assay

Wittrup: TCA Protein Precipitation

Wittrup: TEV Protease Purification and Digestion

Yeast Protocols

Wittrup: Yeast Transformation

Wittrup: Plasmid rescue from yeast

Wittrup: Yeast storage and revival

Wittrup: Yeast surface display

Wittrup: Cell Surface Secretion Assay

Wittrup: Fluorescence Microscopy of Yeast

Wittrup: Yeast Preps for Western Blotting

Cell culture

Wittrup: New Cell Line Banking and Authentication

Wittrup: Basic Culture Media

Wittrup: Recovery Media

Wittrup: Freezing Media

Wittrup: Freezing Protocol

Wittrup: Subculturing Protocol

Wittrup: Thawing Protocol

Wittrup: Crystal Violet Toxicity Assay Protocol

Wittrup: HEK293-F Transfection

Wittrup: Mycoplasma Detection and Clearance

Animal studies

Wittrup:Immunohistochemistry of frozen sections

Wittrup:Immunohistochemistry of paraffin embedded sections

Wittrup:Immunofluorescence of frozen sections

Wittrup:Tumor dissociation

Wittrup:Making a cell line from tumor sections

Individual Lab Member Protocols

User: Cary F Opel

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