Wittrup:Protocols: Difference between revisions
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=== | ===General Lab Duties=== | ||
[[Wittrup: | [[Wittrup: Dishwashing]] | ||
===Recipes=== | |||
[[Wittrup: Buffers]] | |||
[[Wittrup: Yeast Media/Buffers]] | |||
[[Wittrup: E. coli Media]] | |||
===Equipment=== | |||
[[Wittrup:XL Flow cytometer]] | |||
[[Wittrup:Deltavision deconvolution microscope]] | |||
===DNA=== | ===DNA=== | ||
[[Wittrup: Plasmid | [[Wittrup: Plasmid miniprep]] | ||
[[Wittrup: Gel electrophoresis | [[Wittrup: Restriction digestion]] | ||
[[Wittrup: Gel electrophoresis]] | |||
[[Wittrup: Gel extraction]] | |||
[[Wittrup: Site directed mutagenesis by QuikChange]] | [[Wittrup: Site directed mutagenesis by QuikChange]] | ||
| Line 18: | Line 31: | ||
[[Wittrup: PCR]] | [[Wittrup: PCR]] | ||
[[Wittrup: Yeast colony PCR]] | |||
===Proteins=== | ===Proteins=== | ||
| Line 26: | Line 39: | ||
[[Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)]] | [[Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)]] | ||
[[Wittrup:FPLC general use]] | [[Wittrup: FPLC general use]] | ||
[[Wittrup:SDS-PAGE]] | [[Wittrup: SDS-PAGE]] | ||
[[Wittrup:ECL Western Blots and Phosphorimager]] | [[Wittrup: ECL Western Blots and Phosphorimager]] | ||
[[Wittrup:Protein concentration by ultrafiltration]] | [[Wittrup: Protein concentration by ultrafiltration]] | ||
[[Wittrup: Protein conjugation with fluorophores]] | |||
[[Wittrup: Fluorescein Quench Titration Assay]] | |||
[[Wittrup: TCA Protein Precipitation]] | |||
[[Wittrup: TEV Protease Purification and Digestion]] | |||
===Yeast Protocols=== | ===Yeast Protocols=== | ||
| Line 44: | Line 64: | ||
[[Wittrup: Yeast surface display]] | [[Wittrup: Yeast surface display]] | ||
[[Wittrup: Cell Surface Secretion Assay]] | |||
[[Wittrup: Fluorescence Microscopy of Yeast]] | |||
[[Wittrup: Yeast Preps for Western Blotting]] | |||
===Cell culture=== | ===Cell culture=== | ||
=== | [[Wittrup: New Cell Line Banking and Authentication]] | ||
[[Wittrup: Basic Culture Media]] | |||
[[Wittrup: Recovery Media]] | |||
[[Wittrup: Freezing Media]] | |||
[[Wittrup: Freezing Protocol]] | |||
[[Wittrup: Subculturing Protocol]] | |||
[[Wittrup: Thawing Protocol]] | |||
[[Wittrup: Crystal Violet Toxicity Assay Protocol]] | |||
[[Wittrup: HEK293-F Transfection]] | |||
[[Wittrup: Mycoplasma Detection and Clearance]] | |||
===Animal studies=== | |||
[[Wittrup:Immunohistochemistry of frozen sections]] | |||
[[Wittrup:Immunohistochemistry of paraffin embedded sections]] | |||
[[Wittrup:Immunofluorescence of frozen sections]] | |||
[[Wittrup: | [[Wittrup:Tumor dissociation]] | ||
[[Wittrup: | [[Wittrup:Making a cell line from tumor sections]] | ||
===Individual Lab Member Protocols=== | |||
[[ | [[User: Cary F Opel]] | ||
Revision as of 10:59, 16 August 2012
General Lab Duties
Recipes
Equipment
Wittrup:Deltavision deconvolution microscope
DNA
Wittrup: Restriction digestion
Wittrup: Site directed mutagenesis by QuikChange
Proteins
Wittrup: Determining Protein Concentration
Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)
Wittrup: ECL Western Blots and Phosphorimager
Wittrup: Protein concentration by ultrafiltration
Wittrup: Protein conjugation with fluorophores
Wittrup: Fluorescein Quench Titration Assay
Wittrup: TCA Protein Precipitation
Wittrup: TEV Protease Purification and Digestion
Yeast Protocols
Wittrup: Plasmid rescue from yeast
Wittrup: Yeast storage and revival
Wittrup: Yeast surface display
Wittrup: Cell Surface Secretion Assay
Wittrup: Fluorescence Microscopy of Yeast
Wittrup: Yeast Preps for Western Blotting
Cell culture
Wittrup: New Cell Line Banking and Authentication
Wittrup: Subculturing Protocol
Wittrup: Crystal Violet Toxicity Assay Protocol
Wittrup: HEK293-F Transfection
Wittrup: Mycoplasma Detection and Clearance
Animal studies
Wittrup:Immunohistochemistry of frozen sections
Wittrup:Immunohistochemistry of paraffin embedded sections
Wittrup:Immunofluorescence of frozen sections
Wittrup:Making a cell line from tumor sections
