Wittrup:Protocols
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Current revision (13:59, 16 August 2012) (view source) (→Animal studies) |
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| + | ===General Lab Duties=== | ||
| + | |||
| + | [[Wittrup: Dishwashing]] | ||
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===Recipes=== | ===Recipes=== | ||
| - | [[Wittrup:Buffers | + | [[Wittrup: Buffers]] |
| + | |||
| + | [[Wittrup: Yeast Media/Buffers]] | ||
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| + | [[Wittrup: E. coli Media]] | ||
===Equipment=== | ===Equipment=== | ||
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===DNA=== | ===DNA=== | ||
| - | [[Wittrup: Plasmid | + | [[Wittrup: Plasmid miniprep]] |
| - | [[Wittrup: Gel electrophoresis | + | [[Wittrup: Restriction digestion]] |
| + | |||
| + | [[Wittrup: Gel electrophoresis]] | ||
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| + | [[Wittrup: Gel extraction]] | ||
[[Wittrup: Site directed mutagenesis by QuikChange]] | [[Wittrup: Site directed mutagenesis by QuikChange]] | ||
[[Wittrup: PCR]] | [[Wittrup: PCR]] | ||
| + | |||
| + | [[Wittrup: Yeast colony PCR]] | ||
===Proteins=== | ===Proteins=== | ||
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[[Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)]] | [[Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)]] | ||
| - | [[Wittrup:FPLC general use]] | + | [[Wittrup: FPLC general use]] |
| - | [[Wittrup:SDS-PAGE]] | + | [[Wittrup: SDS-PAGE]] |
| - | [[Wittrup:ECL Western Blots and Phosphorimager]] | + | [[Wittrup: ECL Western Blots and Phosphorimager]] |
| - | [[Wittrup:Protein concentration by ultrafiltration]] | + | [[Wittrup: Protein concentration by ultrafiltration]] |
| - | [[Wittrup:Protein conjugation with fluorophores]] | + | [[Wittrup: Protein conjugation with fluorophores]] |
| + | |||
| + | [[Wittrup: Fluorescein Quench Titration Assay]] | ||
| + | |||
| + | [[Wittrup: TCA Protein Precipitation]] | ||
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| + | [[Wittrup: TEV Protease Purification and Digestion]] | ||
===Yeast Protocols=== | ===Yeast Protocols=== | ||
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[[Wittrup: Yeast surface display]] | [[Wittrup: Yeast surface display]] | ||
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| + | [[Wittrup: Cell Surface Secretion Assay]] | ||
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| + | [[Wittrup: Fluorescence Microscopy of Yeast]] | ||
| + | |||
| + | [[Wittrup: Yeast Preps for Western Blotting]] | ||
===Cell culture=== | ===Cell culture=== | ||
| - | [[Wittrup: Cell | + | [[Wittrup: New Cell Line Banking and Authentication]] |
| + | |||
| + | [[Wittrup: Basic Culture Media]] | ||
| + | |||
| + | [[Wittrup: Recovery Media]] | ||
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| + | [[Wittrup: Freezing Media]] | ||
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| + | [[Wittrup: Freezing Protocol]] | ||
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| + | [[Wittrup: Subculturing Protocol]] | ||
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| + | [[Wittrup: Thawing Protocol]] | ||
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| + | [[Wittrup: Crystal Violet Toxicity Assay Protocol]] | ||
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| + | [[Wittrup: HEK293-F Transfection]] | ||
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| + | [[Wittrup: Mycoplasma Detection and Clearance]] | ||
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| + | ===Animal studies=== | ||
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| + | [[Wittrup:Immunohistochemistry of frozen sections]] | ||
| + | |||
| + | [[Wittrup:Immunohistochemistry of paraffin embedded sections]] | ||
| - | + | [[Wittrup:Immunofluorescence of frozen sections]] | |
| - | [[Wittrup: | + | [[Wittrup:Tumor dissociation]] |
| - | [[Wittrup: | + | [[Wittrup:Making a cell line from tumor sections]] |
| - | + | ===Individual Lab Member Protocols=== | |
| - | [[ | + | [[User: Cary F Opel]] |
Current revision
Contents |
General Lab Duties
Recipes
Equipment
Wittrup:Deltavision deconvolution microscope
DNA
Wittrup: Restriction digestion
Wittrup: Site directed mutagenesis by QuikChange
Proteins
Wittrup: Determining Protein Concentration
Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)
Wittrup: ECL Western Blots and Phosphorimager
Wittrup: Protein concentration by ultrafiltration
Wittrup: Protein conjugation with fluorophores
Wittrup: Fluorescein Quench Titration Assay
Wittrup: TCA Protein Precipitation
Wittrup: TEV Protease Purification and Digestion
Yeast Protocols
Wittrup: Plasmid rescue from yeast
Wittrup: Yeast storage and revival
Wittrup: Yeast surface display
Wittrup: Cell Surface Secretion Assay
Wittrup: Fluorescence Microscopy of Yeast
Wittrup: Yeast Preps for Western Blotting
Cell culture
Wittrup: New Cell Line Banking and Authentication
Wittrup: Subculturing Protocol
Wittrup: Crystal Violet Toxicity Assay Protocol
Wittrup: HEK293-F Transfection
Wittrup: Mycoplasma Detection and Clearance
Animal studies
Wittrup:Immunohistochemistry of frozen sections
Wittrup:Immunohistochemistry of paraffin embedded sections
Wittrup:Immunofluorescence of frozen sections
Wittrup:Making a cell line from tumor sections
