Wittrup:Protocols

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+===General Lab Duties===
+[[Wittrup: Dishwashing]]
 ===Recipes===
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 [[Wittrup:  PCR]]
+[[Wittrup:  Yeast colony PCR]]
 ===Proteins===
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 [[Wittrup:  TCA Protein Precipitation]]
+[[Wittrup:  TEV Protease Purification and Digestion]]
 ===Yeast Protocols===
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 ===Cell culture===
+[[Wittrup: New Cell Line Banking and Authentication]]
 [[Wittrup: Basic Culture Media]]
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 [[Wittrup: Thawing Protocol]]
+[[Wittrup: Crystal Violet Toxicity Assay Protocol]]
+[[Wittrup: HEK293-F Transfection]]
+[[Wittrup: Mycoplasma Detection and Clearance]]
+===Animal studies===
+[[Wittrup:Immunohistochemistry of frozen sections]]
+[[Wittrup:Immunohistochemistry of paraffin embedded sections]]
+[[Wittrup:Immunofluorescence of frozen sections]]
+[[Wittrup:Tumor dissociation]]
+[[Wittrup:Making a cell line from tumor sections]]
+===Individual Lab Member Protocols===
+[[User: Cary F Opel]]

Current revision