Wittrup:Protocols: Difference between revisions
Xiaosai Yao (talk | contribs) No edit summary |
Xiaosai Yao (talk | contribs) |
||
Line 90: | Line 90: | ||
===Animal studies=== | ===Animal studies=== | ||
[[Immunohistochemistry of frozen sections]] | |||
[[Immunohistochemistry of paraffin embedded sections]] | |||
[[Immunofluorescence of frozen sections]] | |||
[[Immunofluorescence of paraffin embedded sections]] | |||
[[Tumor dissociation]] |
Revision as of 16:29, 6 October 2009
Recipes
Equipment
Wittrup:Deltavision deconvolution microscope
DNA
Wittrup: Restriction digestion
Wittrup: Site directed mutagenesis by QuikChange
Proteins
Wittrup: Determining Protein Concentration
Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)
Wittrup: ECL Western Blots and Phosphorimager
Wittrup: Protein concentration by ultrafiltration
Wittrup: Protein conjugation with fluorophores
Wittrup: Fluorescein Quench Titration Assay
Wittrup: TCA Protein Precipitation
Wittrup: TEV Protease Purification and Digestion
Yeast Protocols
Wittrup: Plasmid rescue from yeast
Wittrup: Yeast storage and revival
Wittrup: Yeast surface display
Wittrup: Cell Surface Secretion Assay
Wittrup: Fluorescence Microscopy of Yeast
Wittrup: Yeast Preps for Western Blotting
Cell culture
Wittrup: New Cell Line Banking and Authentication
Wittrup: Subculturing Protocol
Wittrup: Crystal Violet Toxicity Assay Protocol
Wittrup: HEK293-F Transfection
Wittrup: Mycoplasma Detection and Clearance
Animal studies
Immunohistochemistry of frozen sections Immunohistochemistry of paraffin embedded sections Immunofluorescence of frozen sections Immunofluorescence of paraffin embedded sections Tumor dissociation