Wittrup:Protocols

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===Software===
===Software===
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[[Wittrup:  Sequence alignment]]
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[[Wittrup:  PCR primer design]]
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[[Wittrup:  Protein visualization (PyMOL)]]
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[[Wittrup:  Biology Workbench]]

Revision as of 19:37, 15 January 2006

Contents

Buffers/Equipment/Media

Wittrup:Buffers

Wittrup:Yeast media

Assays

DNA

RNA

Proteins

Wittrup: Determining Protein Concentration

Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)

Wittrup:FPLC general use

Wittrup:SDS-PAGE

Wittrup:ECL Western Blots and Phosphorimager

Wittrup:Protein concentration by ultrafiltration


Yeast Protocols

Wittrup: Yeast Transformation

Wittrup: Plasmid rescue from yeast

Wittrup: Yeast storage and revival

Wittrup: Yeast surface display

Cell culture

Software

Wittrup: Sequence alignment

Wittrup: PCR primer design

Wittrup: Protein visualization (PyMOL)

Wittrup: Biology Workbench

Personal tools