Wittrup:Protocols: Difference between revisions
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[[Wittrup:Yeast media]] | [[Wittrup:Yeast media]] | ||
[[Wittrup:XL Flow cytometer]] | |||
[[Wittrup:Deltavision deconvolution microscope]] | |||
===DNA=== | ===DNA=== | ||
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[[Wittrup: PCR]] | [[Wittrup: PCR]] | ||
===Proteins=== | ===Proteins=== | ||
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[[Wittrup:Protein concentration by ultrafiltration]] | [[Wittrup:Protein concentration by ultrafiltration]] | ||
[[Wittrup:Protein conjugation with fluorophores]] | |||
===Yeast Protocols=== | ===Yeast Protocols=== | ||
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===Cell culture=== | ===Cell culture=== | ||
[[Wittrup: Cell culture protocols]] | |||
===Software=== | ===Software=== | ||
Revision as of 17:42, 15 January 2006
Buffers/Equipment/Media
Wittrup:Deltavision deconvolution microscope
DNA
Wittrup: Plasmid minipreps, restriction digestion
Wittrup: Gel electrophoresis and band purification
Wittrup: Site directed mutagenesis by QuikChange
Proteins
Wittrup: Determining Protein Concentration
Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)
Wittrup:ECL Western Blots and Phosphorimager
Wittrup:Protein concentration by ultrafiltration
Wittrup:Protein conjugation with fluorophores
Yeast Protocols
Wittrup: Plasmid rescue from yeast
Wittrup: Yeast storage and revival
Wittrup: Yeast surface display
Cell culture
Wittrup: Cell culture protocols
