Wittrup:Protocols

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(DNA)
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[[Wittrup:Yeast media]]
[[Wittrup:Yeast media]]
-
===Assays===
+
[[Wittrup:XL Flow cytometer]]
 +
[[Wittrup:Deltavision deconvolution microscope]]
===DNA===
===DNA===
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[[Wittrup:  PCR]]
[[Wittrup:  PCR]]
-
 
-
===RNA===
 
===Proteins===
===Proteins===
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[[Wittrup:Protein concentration by ultrafiltration]]
[[Wittrup:Protein concentration by ultrafiltration]]
 +
[[Wittrup:Protein conjugation with fluorophores]]
===Yeast Protocols===
===Yeast Protocols===
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===Cell culture===
===Cell culture===
 +
 +
[[Wittrup: Cell culture protocols]]
===Software===
===Software===

Revision as of 19:42, 15 January 2006

Contents

Buffers/Equipment/Media

Wittrup:Buffers

Wittrup:Yeast media

Wittrup:XL Flow cytometer

Wittrup:Deltavision deconvolution microscope

DNA

Wittrup: Plasmid minipreps, restriction digestion

Wittrup: Gel electrophoresis and band purification

Wittrup: Site directed mutagenesis by QuikChange

Wittrup: PCR

Proteins

Wittrup: Determining Protein Concentration

Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)

Wittrup:FPLC general use

Wittrup:SDS-PAGE

Wittrup:ECL Western Blots and Phosphorimager

Wittrup:Protein concentration by ultrafiltration

Wittrup:Protein conjugation with fluorophores

Yeast Protocols

Wittrup: Yeast Transformation

Wittrup: Plasmid rescue from yeast

Wittrup: Yeast storage and revival

Wittrup: Yeast surface display

Cell culture

Wittrup: Cell culture protocols

Software

Wittrup: Sequence alignment

Wittrup: PCR primer design

Wittrup: Protein visualization (PyMOL)

Wittrup: Biology Workbench

Personal tools