Wittrup:Protocols
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[[Wittrup:XL Flow cytometer]] | [[Wittrup:XL Flow cytometer]] | ||
Revision as of 20:43, 15 January 2006
Contents |
Recipes
Equipment
Wittrup:Deltavision deconvolution microscope
DNA
Wittrup: Plasmid minipreps, restriction digestion
Wittrup: Gel electrophoresis and band purification
Wittrup: Site directed mutagenesis by QuikChange
Proteins
Wittrup: Determining Protein Concentration
Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)
Wittrup:ECL Western Blots and Phosphorimager
Wittrup:Protein concentration by ultrafiltration
Wittrup:Protein conjugation with fluorophores
Yeast Protocols
Wittrup: Plasmid rescue from yeast
Wittrup: Yeast storage and revival
Wittrup: Yeast surface display
Cell culture
Wittrup: Cell culture protocols
