Wittrup:Protocols

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===Buffers/Equipment/Media===
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===Recipes===
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[[Wittrup:Buffers]]
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[[Wittrup:Buffers and media]]
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[[Wittrup:Yeast media]]
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===Equipment===
[[Wittrup:XL Flow cytometer]]
[[Wittrup:XL Flow cytometer]]

Revision as of 19:43, 15 January 2006

Contents

Recipes

Wittrup:Buffers and media

Equipment

Wittrup:XL Flow cytometer

Wittrup:Deltavision deconvolution microscope

DNA

Wittrup: Plasmid minipreps, restriction digestion

Wittrup: Gel electrophoresis and band purification

Wittrup: Site directed mutagenesis by QuikChange

Wittrup: PCR

Proteins

Wittrup: Determining Protein Concentration

Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)

Wittrup:FPLC general use

Wittrup:SDS-PAGE

Wittrup:ECL Western Blots and Phosphorimager

Wittrup:Protein concentration by ultrafiltration

Wittrup:Protein conjugation with fluorophores

Yeast Protocols

Wittrup: Yeast Transformation

Wittrup: Plasmid rescue from yeast

Wittrup: Yeast storage and revival

Wittrup: Yeast surface display

Cell culture

Wittrup: Cell culture protocols

Software

Wittrup: Sequence alignment

Wittrup: PCR primer design

Wittrup: Protein visualization (PyMOL)

Wittrup: Biology Workbench

Personal tools