Wittrup:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 75: Line 75:
[[Wittrup: Thawing Protocol]]
[[Wittrup: Thawing Protocol]]
-
 
-
===Software===
 
-
 
-
[[Wittrup:  Sequence alignment]]
 
-
 
-
[[Wittrup:  PCR primer design]]
 
-
 
-
[[Wittrup:  Protein visualization (PyMOL)]]
 
-
 
-
[[Wittrup:  Biology Workbench]]
 

Revision as of 13:35, 23 September 2006

Contents

Recipes

Wittrup: Buffers

Wittrup: Yeast Media/Buffers

Wittrup: E. coli Media

Equipment

Wittrup:XL Flow cytometer

Wittrup:Deltavision deconvolution microscope

DNA

Wittrup: Plasmid miniprep

Wittrup: Restriction digestion

Wittrup: Gel electrophoresis

Wittrup: Gel extraction

Wittrup: Site directed mutagenesis by QuikChange

Wittrup: PCR

Proteins

Wittrup: Determining Protein Concentration

Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)

Wittrup: FPLC general use

Wittrup: SDS-PAGE

Wittrup: ECL Western Blots and Phosphorimager

Wittrup: Protein concentration by ultrafiltration

Wittrup: Protein conjugation with fluorophores

Wittrup: Fluorescein Quench Titration Assay

Wittrup: TCA Protein Precipitation

Yeast Protocols

Wittrup: Yeast Transformation

Wittrup: Plasmid rescue from yeast

Wittrup: Yeast storage and revival

Wittrup: Yeast surface display

Wittrup: Cell Surface Secretion Assay

Wittrup: Fluorescence Microscopy of Yeast

Cell culture

Wittrup: Basic Culture Media

Wittrup: Recovery Media

Wittrup: Freezing Media

Wittrup: Freezing Protocol

Wittrup: Subculturing Protocol

Wittrup: Cell Counting Protocol

Wittrup: Thawing Protocol

Personal tools