Wittrup: Site directed mutagenesis by QuikChange: Difference between revisions
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* Falcon(R) 2059 polypropylene tubes (15 mL) | * Falcon(R) 2059 polypropylene tubes (15 mL) | ||
* beads for top of reactions | * beads for top of reactions | ||
* [[Wittrup: | * [[Wittrup: E. coli Media|SOC Broth]] | ||
* [[Wittrup: | * [[Wittrup: E. coli Media|LB/Amp Plates]] | ||
Revision as of 12:30, 10 February 2006
Site direted mutagenesis by QuikChange
Materials
- PfuTurbo(TM) DNA polymerase (2.5 U/uL)
- 10x reaction buffer
- Dpn I restriction enzyme (10 U/uL)
- dNTP mix
- Epicurian Coli(R) XL1-Blue supercompetent cells
- your primer 1
- your primer 2
- your plasmid
- Falcon(R) 2059 polypropylene tubes (15 mL)
- beads for top of reactions
- SOC Broth
- LB/Amp Plates
Protocol
A
1. in progress
2.
