Wittrup: Site directed mutagenesis by QuikChange

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Site directed mutagenesis by QuikChange

Materials

  • PfuTurbo(TM) DNA polymerase (2.5 U/uL)
  • 10x reaction buffer
  • Dpn I restriction enzyme (10 U/uL)
  • dNTP mix
  • Epicurian Coli(R) XL1-Blue supercompetent cells


  • your primer 1
  • your primer 2
  • your plasmid



Protocol

Use protocol for Stratagene's Quikchange mutagenesis. Manual at: [1]

You will have to register to access this protocol.


Protocol notes:

- Add extra extension time to increase efficiency (1.5 to 2 min per kb). - Can do multiple QC's simultaneously by adding both primers to the PCR and sequencing more clones.


See other community protocols for this: Site-directed mutagenesis

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