Wittrup: Site directed mutagenesis by QuikChange

Site direted mutagenesis by QuikChange

Materials

• PfuTurbo(TM) DNA polymerase (2.5 U/uL)
• 10x reaction buffer
• Dpn I restriction enzyme (10 U/uL)
• dNTP mix
• Epicurian Coli(R) XL1-Blue supercompetent cells

• your primer 1
• your primer 2
• your plasmid

• Falcon(R) 2059 polypropylene tubes (15 mL)
• beads for top of reactions
• SOC Broth
• LB/Amp Plates

Protocol

under development.

See other community protocols for this.