Xwang:Research: Difference between revisions
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== '''PROJECT 2. Chromatin and epigenomic landscape of the developing maize endosperm''' == | == '''PROJECT 2. Chromatin and epigenomic landscape of the developing maize endosperm''' == | ||
<div> Another research interest in our Lab is to understand the epigenomic regulatory mechanisms that function during early maize endosperm development. In collaboration with Dr. Ramin Yadegari's Lab, We are currently producing selected histone marks and DNA methylation using Illumina sequencing in a famous maize hybrid B73 cross Mo17. Another potential direction we are looking for is to study if the epigenetic genomic imprinting influence the heterosis phenomena. | |||
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Revision as of 18:22, 12 March 2011
PROJECT 1. Regulation of early endosperm development in maize
We first conducted a in silico search of maize annotated genes in its 5.0a version in plant transcription factor database, and identify hundreds potential TF maize genes. Then we utilized publish gene microarray expression data and our self-produced RNA-seq data to further filter the gene sets. Our efforts identified ~200 candidate genes that specifically expressed in early stages of endosperm development (A). We are currently performing experimental validation of selected candidate TFs. In the next step, to construct the regulatory network of those identified TFs, we identified the genes whose expression highly correlated with those TFs and calculated their topological structures (B). The regulatory network is finally illustrated in (C). Additionally, we are developing hidden markov model (HMM) to detect the alternative splicing genes during the development (D).
PROJECT 2. Chromatin and epigenomic landscape of the developing maize endosperm