Xylene cyanol: Difference between revisions
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'''Xylene cyanol''' is most commonly used as an [[agarose gel electrophoresis]] size marker. | [[Image:Agarose gel dyes.jpg|right|200px]] | ||
'''Xylene cyanol''' (XC) is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than [[bromophenol blue]] (670g/mol) because of its charge (1 net negative charge). | |||
* 10'000 bp at 0.7% | |||
* 4000 bp at 1.5% | |||
* 750 bp at 2.0% | |||
* 200 bp at 3.0% | |||
== order information == | |||
* CAS 2650-17-1 | |||
* e.g. [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=X4126|SIGMA&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC Sigma Xylene Cyanol FF X4126] 10g at around €50 | |||
== see also == | |||
* [[agarose gel loading buffer]] | |||
* [[agarose gel electrophoresis]] | |||
* [[Cresol Red]] - faster than XC | |||
* [[Bromophenol blue]] - faster than XC | |||
== external links == | |||
* [http://en.wikipedia.org/wiki/Xylene_cyanol Xylene cyanol in the wikipedia] | |||
[[Category:Material]] [[Category:Agarose gel electrophoresis]] [[Category:Dye]] | |||
Latest revision as of 07:20, 3 June 2009
Xylene cyanol (XC) is most commonly used as an agarose gel electrophoresis size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than bromophenol blue (670g/mol) because of its charge (1 net negative charge).
- 10'000 bp at 0.7%
- 4000 bp at 1.5%
- 750 bp at 2.0%
- 200 bp at 3.0%
order information
- CAS 2650-17-1
- e.g. Sigma Xylene Cyanol FF X4126 10g at around €50
see also
- agarose gel loading buffer
- agarose gel electrophoresis
- Cresol Red - faster than XC
- Bromophenol blue - faster than XC
