Xylene cyanol: Difference between revisions
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'''Xylene cyanol''' (XC) is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than [[bromophenol blue]] (670g/mol) because of its charge (1 net negative charge). | '''Xylene cyanol''' (XC) is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than [[bromophenol blue]] (670g/mol) because of its charge (1 net negative charge). | ||
* | * 10'000 bp at 0.7% | ||
* 4000 bp at 1.5% | * 4000 bp at 1.5% | ||
* 750 bp at 2.0% | * 750 bp at 2.0% | ||
Latest revision as of 07:20, 3 June 2009
Xylene cyanol (XC) is most commonly used as an agarose gel electrophoresis size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than bromophenol blue (670g/mol) because of its charge (1 net negative charge).
- 10'000 bp at 0.7%
- 4000 bp at 1.5%
- 750 bp at 2.0%
- 200 bp at 3.0%
order information
- CAS 2650-17-1
- e.g. Sigma Xylene Cyanol FF X4126 10g at around €50
see also
- agarose gel loading buffer
- agarose gel electrophoresis
- Cresol Red - faster than XC
- Bromophenol blue - faster than XC
