Xylene cyanol - Revision history

Jakob Suckale at 14:20, 3 June 2009

2009-06-03T14:20:09Z

←Older revision		Revision as of 14:20, 3 June 2009
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	'''Xylene cyanol''' (XC) is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than [[bromophenol blue]] (670g/mol) because of its charge (1 net negative charge).		'''Xylene cyanol''' (XC) is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than [[bromophenol blue]] (670g/mol) because of its charge (1 net negative charge).

-	* ~~5000~~ bp at 0.5	+	* 10'000 bp at 0.7%
	* 4000 bp at 1.5%		* 4000 bp at 1.5%
	* 750 bp at 2.0%		* 750 bp at 2.0%

2009-06-03T14:18:30Z

illustration

←Older revision		Revision as of 14:18, 3 June 2009
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		+	[[Image:Agarose gel dyes.jpg\|right\|200px]]
		+
	'''Xylene cyanol''' (XC) is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than [[bromophenol blue]] (670g/mol) because of its charge (1 net negative charge).		'''Xylene cyanol''' (XC) is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than [[bromophenol blue]] (670g/mol) because of its charge (1 net negative charge).

2009-05-05T15:15:07Z

←Older revision		Revision as of 15:15, 5 May 2009
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-	'''Xylene cyanol''' is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel ~~(while it's own~~ molecular weight ~~is around 540g~~/mol).	+	'''Xylene cyanol''' (XC) is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel. Despite its smaller molecular weight of 539g/mol it runs slower than [[bromophenol blue]] (670g/mol) because of its charge (1 net negative charge).

	* 5000 bp at 0.5		* 5000 bp at 0.5

2009-05-05T15:10:25Z

2009-05-05T14:58:02Z

2009-05-05T14:57:07Z

←Older revision		Revision as of 14:57, 5 May 2009
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	== see also ==		== see also ==
		+	* [[agarose gel loading buffer]]
	* [[agarose gel electrophoresis]]		* [[agarose gel electrophoresis]]
	* [[Cresol Red]]		* [[Cresol Red]]

2009-05-05T14:54:58Z

←Older revision		Revision as of 14:54, 5 May 2009
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	'''Xylene cyanol''' is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel (while it's own molecular weight is around 540g/mol).		'''Xylene cyanol''' is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel (while it's own molecular weight is around 540g/mol).

-	* ~~4000-~~5000 bp at 0.5-1.5%	+	* 5000 bp at 0.5
-	* 750 ~~- 200~~ bp at 2.0-3.0%	+	* 4000 bp at 1.5%
		+	* 750 bp at 2.0%
		+	* 200 bp at 3.0%

	== order information ==		== order information ==

2009-05-05T14:52:59Z

content

←Older revision		Revision as of 14:52, 5 May 2009
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-	'''Xylene cyanol''' is most commonly used as an [[agarose gel electrophoresis]] size marker.	+	'''Xylene cyanol''' is most commonly used as an [[agarose gel electrophoresis]] size marker. It runs alongside different DNA length markers depending on the % of the agarose gel (while it's own molecular weight is around 540g/mol).
		+
		+	* 4000-5000 bp at 0.5-1.5%
		+	* 750 - 200 bp at 2.0-3.0%
		+
		+	== order information ==
		+	* CAS 2650-17-1
		+
		+	* e.g. [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=X4126\|SIGMA&N5=SEARCH_CONCAT_PNO\|BRAND_KEY&F=SPEC Sigma Xylene Cyanol FF X4126] 10g at around €50

	== see also ==		== see also ==

2009-05-05T14:42:23Z

links

←Older revision		Revision as of 14:42, 5 May 2009
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	'''Xylene cyanol''' is most commonly used as an [[agarose gel electrophoresis]] size marker.		'''Xylene cyanol''' is most commonly used as an [[agarose gel electrophoresis]] size marker.
		+
		+	== see also ==
		+	* [[agarose gel electrophoresis]]
		+	* [[Cresol Red]]
		+	* [[Bromophenol blue]]
		+
		+	== external links ==
		+	* [http://en.wikipedia.org/wiki/Xylene_cyanol Xylene cyanol in the wikipedia]

2009-05-05T14:38:29Z

lead section

New page

'''Xylene cyanol''' is most commonly used as an [[agarose gel electrophoresis]] size marker.

←Older revision		Revision as of 15:10, 5 May 2009
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	* [[agarose gel loading buffer]]		* [[agarose gel loading buffer]]
	* [[agarose gel electrophoresis]]		* [[agarose gel electrophoresis]]
-	* [[Cresol Red]]	+	* [[Cresol Red]] - faster than XC
-	* [[Bromophenol blue]]	+	* [[Bromophenol blue]] - faster than XC

	== external links ==		== external links ==

←Older revision		Revision as of 14:58, 5 May 2009
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	== external links ==		== external links ==
	* [http://en.wikipedia.org/wiki/Xylene_cyanol Xylene cyanol in the wikipedia]		* [http://en.wikipedia.org/wiki/Xylene_cyanol Xylene cyanol in the wikipedia]
		+
		+	[[Category:Material]] [[Category:Agarose gel electrophoresis]] [[Category:Dye]]