Yeast Colony PCR

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#Using sterile pipette tips, transfer a 1 mm colony into 50 uL of 60 U/ml Zymolase
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''Back to [[Protocols]]''
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#*3 uL of 1 U/mL Zymolase stock solution
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#*47 uL of water
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#Incubate cell suspension at 37 C for 30 minutes.
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#Incubate cell suspension at 95 C for 10 minutes.
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#Use 5 uL of the cell suspension for a 50 uL PCR reaction
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'''Zymolase Stock Solution'''
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==Lab Protocols==
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*10,000 U lyticase (Sigma)
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*[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]
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*1 mL KHPO4 buffer, pH 7.5 (adjusted with KOH)
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*[[Endy:Yeast Colony PCR]]
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*0.2 mL 5M NaCl
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*[[Blackburn:Yeast_Colony_PCR_v2.0|Blackburn Lab:  Quick and Easy Yeast Colony PCR v2.0]]
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*5 mL 100% glycerol
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*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]
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*Add H2O to 10 mL
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*[[BE.109:Bio-material_engineering/PCR_of_gold_binding_candidates| 20.109 lab protocol, steps 2 and 4]]
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*Store at -20 C
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[[Category:Protocol]]
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[[Category:Yeast]]
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[[Category:DNA]]

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Lab Protocols

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