Yeast Colony PCR: Difference between revisions

Latest revision as of 15:59, 20 March 2009

Back to Protocols

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-See also: [[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]
+''Back to [[Protocols]]''
+==Lab Protocols==
+*[[Silver: Colony PCR|Silver Lab: Yeast Colony PCR]]
+*[[Endy:Yeast Colony PCR]]
+*[[Blackburn:Yeast_Colony_PCR_v2.0|Blackburn Lab:  Quick and Easy Yeast Colony PCR v2.0]]
+*[[Smolke:Colony_PCR_tsb|Smolke Lab: Colony PCR (Travis' version)]]
+*[[BE.109:Bio-material_engineering/PCR_of_gold_binding_candidates| 20.109 lab protocol, steps 2 and 4]]
+[[Category:Protocol]]
-#Using sterile pipette tips, transfer a 1 mm colony into 50 uL of 60 U/ml Zymolase
+[[Category:Yeast]]
-#*3 uL of 1 U/mL Zymolase stock solution
+[[Category:DNA]]
-#*47 uL of water
-#Incubate cell suspension at 37 C for 30 minutes.
-#Incubate cell suspension at 95 C for 10 minutes.
-#Use 5 uL of the cell suspension for a 50 uL PCR reaction
-'''Zymolase Stock Solution'''
-*10,000 U lyticase (Sigma)
-*1 mL KHPO4 buffer, pH 7.5 (adjusted with KOH)
-*0.2 mL 5M NaCl
-*5 mL 100% glycerol
-*Add H2O to 10 mL
-*Store at -20 C