Yeast artificial chromosomes: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
mNo edit summary |
||
Line 11: | Line 11: | ||
<biblio> | <biblio> | ||
# Burke87 pmid= 3033825 | # Burke87 pmid=3033825 | ||
# Anand89 pmid=2542900 | # Anand89 pmid=2542900 | ||
# Sgaramella90 pmid=2189412 | # Sgaramella90 pmid=2189412 |
Revision as of 21:23, 17 April 2006
Yeast artificial chromosomes (YACs) are synthetic double stranded linear constructs containing the elements necessary for replication in yeast. These elements are:
- an autonomous replication sequence (ARS)
- a centromere
- a telomeric sequence at each end
Typically the chromosome also contains a selection marker such as Lys2 or Ura3.
A common tool for constructing YACs is a shuttle plasmid such as pYAC4 which replicates in E. coli, has a multiple cloning site, and a pair of telomeres which can be cleaved to form a linear fragment. Available as an E.coli plasmid ATCC 67379, sequence at U01086. Yeast host AB1380 is available as ATCC 204682.
Minimal size for a YAC is between 50kb and 100kb, while maximum sizes are 1Mb to 3Mb.
- Burke DT, Carle GF, and Olson MV. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science. 1987 May 15;236(4803):806-12. DOI:10.1126/science.3033825 |
- Anand R, Villasante A, and Tyler-Smith C. Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis. Nucleic Acids Res. 1989 May 11;17(9):3425-33. DOI:10.1093/nar/17.9.3425 |
- Sgaramella V, Ferretti L, Damiani G, and Sora S. A procedure for cloning restriction fragments of DNA as single inserts in yeast artificial chromosomes. Biochem Int. 1990;20(3):503-10.
- Wells RA, Germino GG, Krishna S, Buckle VJ, and Reeders ST. Telomere-related sequences at interstitial sites in the human genome. Genomics. 1990 Dec;8(4):699-704. DOI:10.1016/0888-7543(90)90257-u |
- Burke DT and Olson MV. Preparation of clone libraries in yeast artificial-chromosome vectors. Methods Enzymol. 1991;194:251-70. DOI:10.1016/0076-6879(91)94020-d |
- Ragoussis J, Trowsdale J, and Markie D. Mitotic recombination of yeast artificial chromosomes. Nucleic Acids Res. 1992 Jun 25;20(12):3135-8. DOI:10.1093/nar/20.12.3135 |
- de Bruin D, Lanzer M, and Ravetch JV. Characterization of yeast artificial chromosomes from Plasmodium falciparum: construction of a stable, representative library and cloning of telomeric DNA fragments. Genomics. 1992 Oct;14(2):332-9. DOI:10.1016/s0888-7543(05)80223-x |
- Kuhn RM and Ludwig RA. Complete sequence of the yeast artificial chromosome cloning vector pYAC4. Gene. 1994 Apr 8;141(1):125-7. DOI:10.1016/0378-1119(94)90139-2 |
- Mahmood A, Kimura T, Takenaka M, and Yoshida K. The construction of mobilizable YACs and their direct conjugative transfer from E. coli to yeasts. Nucleic Acids Symp Ser. 1995(34):45-6.
- Mahmood A, Kimura T, Takenaka M, and Yoshida K. The construction of novel mobilizable YAC plasmids and their behavior during trans-kingdom conjugation between bacteria and yeasts. Genet Anal. 1996 Jul;13(2):25-31. DOI:10.1016/1050-3862(95)00146-8 |
- Cocchia M, Kouprina N, Kim SJ, Larionov V, Schlessinger D, and Nagaraja R. Recovery and potential utility of YACs as circular YACs/BACs. Nucleic Acids Res. 2000 Sep 1;28(17):E81. DOI:10.1093/nar/28.17.e81 |