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Yu:Dropout Media - Revision history
2013-05-19T19:58:57Z
Revision history for this page on the wiki
MediaWiki 1.13.2
http://www.openwetware.org/index.php?title=Yu:Dropout_Media&diff=507891&oldid=prev
Christopher Jackson: Fix
2011-05-07T23:02:01Z
<p>Fix</p>
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<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:02, 7 May 2011</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|8. Combine the 200mL nutrient flask into the 700mL agar flask aseptically</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|8. Combine the 200mL nutrient flask into the 700mL agar flask aseptically</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">Combine solutions</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|9. Add 100mL of sterile 20% Glucose prior to pouring</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|9. Add 100mL of sterile 20% Glucose prior to pouring</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2% Glucose</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|2% Glucose</div></td></tr>
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Christopher Jackson
http://www.openwetware.org/index.php?title=Yu:Dropout_Media&diff=507890&oldid=prev
Christopher Jackson: DO Media
2011-05-07T23:01:23Z
<p>DO Media</p>
<p><b>New page</b></p><div>=Dropout Media=<br />
{{Template:Yu}}<br />
<br />
__TOC__<br />
<br />
===Amino Acid Dropout Powder===<br />
<br />
Mix in a '''dry''' mortar. Grind up thoroughly with a pestle for ~15 minutes. Store in 50mL conical tube.<br />
<br />
{| border="0" cellpadding="0"<br />
|width="200px" valign="top"|ADE<br />
|width="100px" valign="top"|0.25g<br />
|-<br />
|width="200px" valign="top"|LEU<br />
|width="100px" valign="top"|5g<br />
|-<br />
|width="200px" valign="top"|p-aminobenzoic acid<br />
|width="100px" valign="top"|0.1g<br />
|-<br />
|span="2" align="center"|''Add 1g each of the following:''<br />
|-<br />
|width="200px" valign="top"|ALA<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|ARG<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|ASN<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|ASP<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|CYS<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|GLN<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|GLU<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|GLY<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|HIS<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|ILE<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|Innositol<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|LYS<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|MET<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|PHE<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|PRO<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|SER<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|THR<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|TRP<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|TYR<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|Uracil<br />
|width="100px" valign="top"|1g<br />
|-<br />
|width="200px" valign="top"|VAL<br />
|width="100px" valign="top"|1g<br />
|}<br />
<br />
----<br />
<br />
===Dropout Liquid Media===<br />
<br />
{| border="0" cellpadding="10"<br />
|+'''Solution recipe for 1L <span id="liquid">liquid AA dropout</span> media'''<br />
|width="600px" valign="top"|1. Measure 800mL millipore water into a 2L plastic beaker.<br />
|width="100px" valign="top"|800mL H<sub>2</sub>O<br />
|-<br />
|2. Weigh 6.7g Yeast Nitrogen Base (w/o Amino Acids) and add it to the mixture<br />
|6.7g Nitrogen Base<br />
|-<br />
|3. Weigh 2g of Amino Acid Dropout Powder and add it to the mixture <br />
|2g AA DO Powder<br />
|-<br />
|4. Stir until completely dissolved<br />
|<br />
|-<br />
|5. Bring solution to 900mL with millipore water in a graduated cylinder<br />
|Volume to 900mL<br />
|-<br />
|6. Autoclave 30 minutes to sterilize<br />
|<br />
|-<br />
|7. Add 100mL of sterile 20% Glucose prior to use<br />
|2% Glucose<br />
|}<br />
<br />
----<br />
<br />
===Dropout Plate Media===<br />
<br />
{| border="0" cellpadding="10"<br />
|+'''Solution recipe for 1L <span id="liquid">agar plate AA dropout</span> media'''<br />
|width="600px" valign="top"|1. Measure 20g Bacto Agar into a 2L flask.<br />
|width="100px" valign="top"|2% w/v Agar<br />
|-<br />
|2. Pour 700mL millipore H<sub>2</sub>O into the flask containing agar<br />
|700mL H<sub>2</sub>O<br />
|-<br />
|span="2" align="center"|''Add the following reagents to a separate beaker''<br />
|-<br />
|3. Add 150mL millipore H<sub>2</sub>O to a separate beaker <br />
|150mL H<sub>2</sub>O<br />
|-<br />
|4. Weigh 6.7g Yeast Nitrogen Base (w/o Amino Acids) and add it to the beaker<br />
|6.7g Nitrogen Base<br />
|-<br />
|5. Weigh 2g of Amino Acid Dropout Powder and add it to the beaker<br />
|2g AA DO Powder<br />
|-<br />
|6. Bring volume in beaker to 200mL and transfer to a 500mL flask<br />
|Volume to 200mL<br />
|-<br />
|7. Autoclave both flasks (nutrients & agar) 30 minutes to sterilize<br />
|<br />
|-<br />
|8. Combine the 200mL nutrient flask into the 700mL agar flask aseptically<br />
|Combine solutions<br />
|-<br />
|9. Add 100mL of sterile 20% Glucose prior to pouring<br />
|2% Glucose<br />
|}</div>
Christopher Jackson