Yu:Genomic prep

1. Inoculate yeast into 10mL YEPD culture and grow overnight to saturation
2. Pellet yeast cells 2 minutes at 2k RPM
3. Discard supernatant and resuspend pellet in 500μL H₂O
4. Transfer yeast suspension to eppendorf tube
5. Pellet yeast cells 2 minutes at 2k RPM
6. Resuspend in 200μL Genomic Detergent Lysis Buffer
7. Add 200μL Phenol:Chloroform:Isoamyl Alcohol (25:24:1)
8. Add ~300mg glass beads
9. Vortex 3-4 minutes
10. Add 200 μL TE₈ and spin down for 5 minutes at maximum speed
11. Transfer the aqueous layer to a new eppendorf tube
12. Add 1mL of 100% Ethanol, mix by inversion, and spin down for 2 minutes at maximum speed at 4°C
13. Discard supernatant
    
    Optional RNAse treatment
    1. Resuspend nucleic acid pellet in 400μL TE₈
    2. Add 3μL of pre-boiled RNAse (10 mg/mL)
    3. Incubate 5 minutes at 37°C
    4. Add 10μL of 4M NH₄OAc and 1mL 100% Ethanol. Mix by inversion and spin down for 2 minutes at maximum speed at 4°C
    5. Discard supernatant
       
       
14. Air-dry pellet
15. Resuspend in 50μL of nuclease-free TE₈