Yu:IPP

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(IPP)
Current revision (15:37, 22 December 2009) (view source)
(fixed)
 
Line 2: Line 2:
{| border="0" cellpadding="10"
{| border="0" cellpadding="10"
|+'''Solution recipe for 500mL <span id="IPP150">IPP150</span>.'''
|+'''Solution recipe for 500mL <span id="IPP150">IPP150</span>.'''
-
|width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into a 500mL beaker for a final concentration of 25mM Tris pH 8.0
+
|width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H<sub>2</sub>O in a 500mL beaker
-
|width="100px" valign="top"|12.5mL 1M Tris pH 8.0
+
|width="100px" valign="top"|25mM Tris-HCl
|-
|-
-
|2. Add 15mL of 5M NaCl for a final concentration of 150mM NaCl
+
|2. Add 15mL of 5M NaCl
-
|15mL 5M NaCl
+
|150mM NaCl
|-
|-
-
|3. Add 5mL of 10% NP-40 for a final concentration of 0.1% NP-40
+
|3. Add 5mL of 10% NP-40
-
|5mL 10% NP-40
+
|0.1% NP-40
|-
|-
|4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder
|4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder
Line 16: Line 16:
{| border="0" cellpadding="10"
{| border="0" cellpadding="10"
|+'''Solution recipe for 500mL <span id="IPP300">IPP300</span>.'''
|+'''Solution recipe for 500mL <span id="IPP300">IPP300</span>.'''
-
|width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into a 500mL beaker for a final concentration of 25mM Tris pH 8.0
+
|width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H<sub>2</sub>O in a 500mL beaker
-
|width="100px" valign="top"|12.5mL 1M Tris pH 8.0
+
|width="100px" valign="top"|25mM Tris-HCl
|-
|-
-
|2. Add 30mL of 5M NaCl for a final concentration of 300mM NaCl
+
|2. Add 30mL of 5M NaCl
-
|30mL 5M NaCl
+
|300mM NaCl
|-
|-
-
|3. Add 5mL of 10% NP-40 for a final concentration of 0.1% NP-40
+
|3. Add 5mL of 10% NP-40
-
|5mL 10% NP-40
+
|0.1% NP-40
|-
|-
|4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder
|4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder
|Volume to 500mL
|Volume to 500mL
|}
|}

Current revision

Home       Procedures       | Lab Members       | Publications       | Research Interests       | Internal Database       Image:UBLogo.jpg


Solution recipe for 500mL IPP150.
1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker 25mM Tris-HCl
2. Add 15mL of 5M NaCl 150mM NaCl
3. Add 5mL of 10% NP-40 0.1% NP-40
4. Bring volume to 500mL with millipore H2O in a graduated cylinder Volume to 500mL
Solution recipe for 500mL IPP300.
1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker 25mM Tris-HCl
2. Add 30mL of 5M NaCl 300mM NaCl
3. Add 5mL of 10% NP-40 0.1% NP-40
4. Bring volume to 500mL with millipore H2O in a graduated cylinder Volume to 500mL
Personal tools