# Yu:IPP

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 Revision as of 14:53, 14 June 2009 (view source) (IPP)← Previous diff Current revision (15:37, 22 December 2009) (view source) (fixed) Line 2: Line 2: {| border="0" cellpadding="10" {| border="0" cellpadding="10" |+'''Solution recipe for 500mL IPP150.''' |+'''Solution recipe for 500mL IPP150.''' - |width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into a 500mL beaker for a final concentration of 25mM Tris pH 8.0 + |width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker - |width="100px" valign="top"|12.5mL 1M Tris pH 8.0 + |width="100px" valign="top"|25mM Tris-HCl |- |- - |2. Add 15mL of 5M NaCl for a final concentration of 150mM NaCl + |2. Add 15mL of 5M NaCl - |15mL 5M NaCl + |150mM NaCl |- |- - |3. Add 5mL of 10% NP-40 for a final concentration of 0.1% NP-40 + |3. Add 5mL of 10% NP-40 - |5mL 10% NP-40 + |0.1% NP-40 |- |- |4. Bring volume to 500mL with millipore H2O in a graduated cylinder |4. Bring volume to 500mL with millipore H2O in a graduated cylinder Line 16: Line 16: {| border="0" cellpadding="10" {| border="0" cellpadding="10" |+'''Solution recipe for 500mL IPP300.''' |+'''Solution recipe for 500mL IPP300.''' - |width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into a 500mL beaker for a final concentration of 25mM Tris pH 8.0 + |width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker - |width="100px" valign="top"|12.5mL 1M Tris pH 8.0 + |width="100px" valign="top"|25mM Tris-HCl |- |- - |2. Add 30mL of 5M NaCl for a final concentration of 300mM NaCl + |2. Add 30mL of 5M NaCl - |30mL 5M NaCl + |300mM NaCl |- |- - |3. Add 5mL of 10% NP-40 for a final concentration of 0.1% NP-40 + |3. Add 5mL of 10% NP-40 - |5mL 10% NP-40 + |0.1% NP-40 |- |- |4. Bring volume to 500mL with millipore H2O in a graduated cylinder |4. Bring volume to 500mL with millipore H2O in a graduated cylinder |Volume to 500mL |Volume to 500mL |} |}

## Current revision

 1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker 25mM Tris-HCl 2. Add 15mL of 5M NaCl 150mM NaCl 3. Add 5mL of 10% NP-40 0.1% NP-40 4. Bring volume to 500mL with millipore H2O in a graduated cylinder Volume to 500mL
 1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker 25mM Tris-HCl 2. Add 30mL of 5M NaCl 300mM NaCl 3. Add 5mL of 10% NP-40 0.1% NP-40 4. Bring volume to 500mL with millipore H2O in a graduated cylinder Volume to 500mL