Yu:IPP: Difference between revisions
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Michaelcyu (talk | contribs) (IPP) |
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{| border="0" cellpadding="10" | {| border="0" cellpadding="10" | ||
|+'''Solution recipe for 500mL <span id="IPP150">IPP150</span>.''' | |+'''Solution recipe for 500mL <span id="IPP150">IPP150</span>.''' | ||
|width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into a 500mL beaker | |width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H<sub>2</sub>O in a 500mL beaker | ||
|width="100px" valign="top"| | |width="100px" valign="top"|25mM Tris-HCl | ||
|- | |- | ||
|2. Add 15mL of 5M | |2. Add 15mL of 5M NaCl | ||
| | |150mM NaCl | ||
|- | |- | ||
|3. Add 5mL of 10% NP-40 | |3. Add 5mL of 10% NP-40 | ||
|0.1% NP-40 | |||
|- | |- | ||
|4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder | |4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder | ||
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{| border="0" cellpadding="10" | {| border="0" cellpadding="10" | ||
|+'''Solution recipe for 500mL <span id="IPP300">IPP300</span>.''' | |+'''Solution recipe for 500mL <span id="IPP300">IPP300</span>.''' | ||
|width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into a 500mL beaker | |width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H<sub>2</sub>O in a 500mL beaker | ||
|width="100px" valign="top"| | |width="100px" valign="top"|25mM Tris-HCl | ||
|- | |- | ||
|2. Add 30mL of 5M | |2. Add 30mL of 5M NaCl | ||
| | |300mM NaCl | ||
|- | |- | ||
|3. Add 5mL of 10% NP-40 | |3. Add 5mL of 10% NP-40 | ||
|0.1% NP-40 | |||
|- | |- | ||
|4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder | |4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder | ||
|Volume to 500mL | |Volume to 500mL | ||
|} | |} | ||
Latest revision as of 12:37, 22 December 2009
| 1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker | 25mM Tris-HCl |
| 2. Add 15mL of 5M NaCl | 150mM NaCl |
| 3. Add 5mL of 10% NP-40 | 0.1% NP-40 |
| 4. Bring volume to 500mL with millipore H2O in a graduated cylinder | Volume to 500mL |
| 1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker | 25mM Tris-HCl |
| 2. Add 30mL of 5M NaCl | 300mM NaCl |
| 3. Add 5mL of 10% NP-40 | 0.1% NP-40 |
| 4. Bring volume to 500mL with millipore H2O in a graduated cylinder | Volume to 500mL |
