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Yu:IPP - Revision history
2013-05-19T13:43:49Z
Revision history for this page on the wiki
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http://www.openwetware.org/index.php?title=Yu:IPP&diff=377500&oldid=prev
Christopher Jackson: fixed
2009-12-22T19:37:23Z
<p>fixed</p>
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<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:37, 22 December 2009</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| border="0" cellpadding="10"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| border="0" cellpadding="10"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Solution recipe for 500mL <span id="IPP150">IPP150</span>.'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Solution recipe for 500mL <span id="IPP150">IPP150</span>.'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into a 500mL beaker <del class="diffchange diffchange-inline">for a final concentration of 25mM Tris pH 8.0</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into <ins class="diffchange diffchange-inline">100mL millipore H<sub>2</sub>O in </ins>a 500mL beaker</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|width="100px" valign="top"|<del class="diffchange diffchange-inline">12.5mL 1M </del>Tris <del class="diffchange diffchange-inline">pH 8.0</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|width="100px" valign="top"|<ins class="diffchange diffchange-inline">25mM </ins>Tris<ins class="diffchange diffchange-inline">-HCl</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|2. Add 15mL of 5M <del class="diffchange diffchange-inline">NaCl for a final concentration of 150mM </del>NaCl</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|2. Add 15mL of 5M NaCl</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">15mL 5M </del>NaCl</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<ins class="diffchange diffchange-inline">150mM </ins>NaCl</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|3. Add 5mL of 10% NP-40 <del class="diffchange diffchange-inline">for a final concentration of </del>0.1<del class="diffchange diffchange-inline">% NP-40</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|3. Add 5mL of 10% NP-40</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|5mL 10</del>% NP-40</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|</ins>0.1% NP-40</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 16:</td>
<td colspan="2" class="diff-lineno">Line 16:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| border="0" cellpadding="10"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| border="0" cellpadding="10"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Solution recipe for 500mL <span id="IPP300">IPP300</span>.'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Solution recipe for 500mL <span id="IPP300">IPP300</span>.'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into a 500mL beaker <del class="diffchange diffchange-inline">for a final concentration of 25mM Tris pH 8.0</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into <ins class="diffchange diffchange-inline">100mL millipore H<sub>2</sub>O in </ins>a 500mL beaker</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|width="100px" valign="top"|<del class="diffchange diffchange-inline">12.5mL 1M </del>Tris <del class="diffchange diffchange-inline">pH 8.0</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|width="100px" valign="top"|<ins class="diffchange diffchange-inline">25mM </ins>Tris<ins class="diffchange diffchange-inline">-HCl</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|2. Add 30mL of 5M <del class="diffchange diffchange-inline">NaCl for a final concentration of 300mM </del>NaCl</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|2. Add 30mL of 5M NaCl</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">30mL 5M </del>NaCl</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<ins class="diffchange diffchange-inline">300mM </ins>NaCl</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|3. Add 5mL of 10% NP-40 <del class="diffchange diffchange-inline">for a final concentration of </del>0.1<del class="diffchange diffchange-inline">% NP-40</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|3. Add 5mL of 10% NP-40</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|5mL 10</del>% NP-40</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">|</ins>0.1% NP-40</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|Volume to 500mL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|Volume to 500mL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<!-- diff generator: internal 2013-05-19 13:43:49 -->
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Christopher Jackson
http://www.openwetware.org/index.php?title=Yu:IPP&diff=314799&oldid=prev
Michaelcyu: IPP
2009-06-14T18:53:50Z
<p>IPP</p>
<p><b>New page</b></p><div>{{Template:Yu}}<br />
{| border="0" cellpadding="10"<br />
|+'''Solution recipe for 500mL <span id="IPP150">IPP150</span>.'''<br />
|width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into a 500mL beaker for a final concentration of 25mM Tris pH 8.0<br />
|width="100px" valign="top"|12.5mL 1M Tris pH 8.0<br />
|-<br />
|2. Add 15mL of 5M NaCl for a final concentration of 150mM NaCl<br />
|15mL 5M NaCl<br />
|-<br />
|3. Add 5mL of 10% NP-40 for a final concentration of 0.1% NP-40<br />
|5mL 10% NP-40<br />
|-<br />
|4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder<br />
|Volume to 500mL<br />
|}<br />
{| border="0" cellpadding="10"<br />
|+'''Solution recipe for 500mL <span id="IPP300">IPP300</span>.'''<br />
|width="600px" valign="top"|1. Measure 12.5mL of 1M Tris pH 8.0 into a 500mL beaker for a final concentration of 25mM Tris pH 8.0<br />
|width="100px" valign="top"|12.5mL 1M Tris pH 8.0<br />
|-<br />
|2. Add 30mL of 5M NaCl for a final concentration of 300mM NaCl<br />
|30mL 5M NaCl<br />
|-<br />
|3. Add 5mL of 10% NP-40 for a final concentration of 0.1% NP-40<br />
|5mL 10% NP-40<br />
|-<br />
|4. Bring volume to 500mL with millipore H<sub>2</sub>O in a graduated cylinder<br />
|Volume to 500mL<br />
|}</div>
Michaelcyu