Yu:IPP

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Solution recipe for 500mL IPP150.
1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker 25mM Tris-HCl
2. Add 15mL of 5M NaCl 150mM NaCl
3. Add 5mL of 10% NP-40 0.1% NP-40
4. Bring volume to 500mL with millipore H2O in a graduated cylinder Volume to 500mL
Solution recipe for 500mL IPP300.
1. Measure 12.5mL of 1M Tris pH 8.0 into 100mL millipore H2O in a 500mL beaker 25mM Tris-HCl
2. Add 30mL of 5M NaCl 300mM NaCl
3. Add 5mL of 10% NP-40 0.1% NP-40
4. Bring volume to 500mL with millipore H2O in a graduated cylinder Volume to 500mL
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