Yu:IPTG: Difference between revisions
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(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for IPTG(Powder stored at -20<sup>0</sup>C)'''. |- |Isopropylthio-β-<sub>D</sub>-galactoside(m.w=238.3) |- |1. Dissol...) |
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Latest revision as of 13:22, 18 September 2009
| Isopropylthio-β-D-galactoside(m.w=238.3) |
| 1. Dissolve a solution of IPTG of 2g of IPTG in 8ml of milipore water H2O |
| 2. Adjust the volume of the solution to 10ml with milipore H2O |
| 3. Sterilize by filtration through a 0.22 micron disposable filter |
| 4. Dispense the solution into 1-ml aliquots and store them at -200C |
