Yu:LiOAc
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(Difference between revisions)
(lioac/te) |
Current revision (17:12, 4 May 2010) (view source) m |
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|+'''Solution recipe for 100mL 5x <span id="TE">LiOAc/TE</span>. Dilute to 1x for use.''' | |+'''Solution recipe for 100mL 5x <span id="TE">LiOAc/TE</span>. Dilute to 1x for use.''' | ||
| - | |width="600px" valign="top"|1. Measure 50mL 1M LiOAC into a 250mL beaker | + | |width="600px" valign="top"|1. Measure 50mL 1M LiOAC into a 250mL beaker |
| - | |width="100px" valign="top"| | + | |width="100px" valign="top"|0.5M LiOAc |
|- | |- | ||
| - | |2. Add 5mL 1M [[Tris]] pH 8.0 | + | |2. Add 5mL 1M [[Tris]] pH 8.0 |
| - | | | + | |50mM TRIS |
|- | |- | ||
| - | |3. Add 2mL .5M [[EDTA]] | + | |3. Add 2mL .5M [[EDTA]] pH 8.0 |
| - | | | + | |10mM EDTA |
|- | |- | ||
|4. Bring volume to 100mL by adding 43mL sterile H<sub>2</sub>O | |4. Bring volume to 100mL by adding 43mL sterile H<sub>2</sub>O | ||
| - | | | + | | |
|- | |- | ||
|5. Sterilize solution with sterile filtering technique | |5. Sterilize solution with sterile filtering technique | ||
| - | | | + | | |
|} | |} | ||
Current revision
| 1. Measure 50mL 1M LiOAC into a 250mL beaker | 0.5M LiOAc |
| 2. Add 5mL 1M Tris pH 8.0 | 50mM TRIS |
| 3. Add 2mL .5M EDTA pH 8.0 | 10mM EDTA |
| 4. Bring volume to 100mL by adding 43mL sterile H2O | |
| 5. Sterilize solution with sterile filtering technique |
