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Yu:LiOAc - Revision history
2013-05-22T00:21:49Z
Revision history for this page on the wiki
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http://www.openwetware.org/index.php?title=Yu:LiOAc&diff=412338&oldid=prev
Christopher Jackson at 21:12, 4 May 2010
2010-05-04T21:12:19Z
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<td colspan='2' style="background-color: white; color:black;">←Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 21:12, 4 May 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| border="0" cellpadding="10"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{| border="0" cellpadding="10"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Solution recipe for 100mL 5x <span id="TE">LiOAc/TE</span>. Dilute to 1x for use.'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|+'''Solution recipe for 100mL 5x <span id="TE">LiOAc/TE</span>. Dilute to 1x for use.'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|width="600px" valign="top"|1. Measure 50mL 1M LiOAC into a 250mL beaker <del class="diffchange diffchange-inline">to bring solution to .5M LiOAc</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|width="600px" valign="top"|1. Measure 50mL 1M LiOAC into a 250mL beaker</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|width="100px" valign="top"|<del class="diffchange diffchange-inline">50mL 1M LiOAC</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|width="100px" valign="top"|<ins class="diffchange diffchange-inline">0.5M LiOAc</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|2. Add 5mL 1M [[Tris]] pH 8.0 <del class="diffchange diffchange-inline">to bring solution to .05M Tris</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|2. Add 5mL 1M [[Tris]] pH 8.0</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">5mL 1M Tris pH 8.0</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<ins class="diffchange diffchange-inline">50mM TRIS</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|3. Add 2mL .5M [[EDTA]] <del class="diffchange diffchange-inline">to bring solution to </del>.<del class="diffchange diffchange-inline">01M EDTA</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|3. Add 2mL .5M [[EDTA]] <ins class="diffchange diffchange-inline">pH 8</ins>.<ins class="diffchange diffchange-inline">0</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">2mL .5M </del>EDTA</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<ins class="diffchange diffchange-inline">10mM </ins>EDTA</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|4. Bring volume to 100mL by adding 43mL sterile H<sub>2</sub>O</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|4. Bring volume to 100mL by adding 43mL sterile H<sub>2</sub>O</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">Volume to 100mL with 43mL H<sub>2</sub>O</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|5. Sterilize solution with sterile filtering technique</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|5. Sterilize solution with sterile filtering technique</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">Sterile filter</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
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Christopher Jackson
http://www.openwetware.org/index.php?title=Yu:LiOAc&diff=314728&oldid=prev
Michaelcyu: lioac/te
2009-06-14T15:48:57Z
<p>lioac/te</p>
<p><b>New page</b></p><div>{{Template:Yu}}<br />
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{| border="0" cellpadding="10"<br />
|+'''Solution recipe for 100mL 5x <span id="TE">LiOAc/TE</span>. Dilute to 1x for use.'''<br />
|width="600px" valign="top"|1. Measure 50mL 1M LiOAC into a 250mL beaker to bring solution to .5M LiOAc<br />
|width="100px" valign="top"|50mL 1M LiOAC<br />
|-<br />
|2. Add 5mL 1M [[Tris]] pH 8.0 to bring solution to .05M Tris<br />
|5mL 1M Tris pH 8.0<br />
|-<br />
|3. Add 2mL .5M [[EDTA]] to bring solution to .01M EDTA<br />
|2mL .5M EDTA<br />
|-<br />
|4. Bring volume to 100mL by adding 43mL sterile H<sub>2</sub>O<br />
|Volume to 100mL with 43mL H<sub>2</sub>O<br />
|-<br />
|5. Sterilize solution with sterile filtering technique<br />
|Sterile filter<br />
|}</div>
Michaelcyu