Yu:Northern solns: Difference between revisions
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(Added Northern solution recipes) |
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{| border="1" cellpadding="10" cellspacing="0" | {| border="1" cellpadding="10" cellspacing="0" | ||
| | |!'''20x <span id="SSC">SSC</span>''' | ||
|width="300px" valign="top"|'''Final Concentration''' | |width="300px" valign="top"|'''Final Concentration''' | ||
|width="100px" valign="top"|'''For 1 Liter''' | |width="100px" valign="top"|'''For 1 Liter''' | ||
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{| border="1" cellpadding="10" cellspacing="0" | {| border="1" cellpadding="10" cellspacing="0" | ||
| | |!'''<span id="10x MOPS">10x MOPS</span>''' | ||
|width="300px" valign="top"|'''Final Concentration''' | |width="300px" valign="top"|'''Final Concentration''' | ||
|width="100px" valign="top"|'''For 500 mL''' | |width="100px" valign="top"|'''For 500 mL''' | ||
Revision as of 12:34, 10 September 2009
Northern Solutions
| !20x SSC | Final Concentration | For 1 Liter | For 4 Liters |
| 3M NaCl | 175.3 g | 701.2 g | |
| 0.3M NaCitrate | 88.2 g | 352.8 g | |
| milliQ H2O | to 1 L | to 4 L |
| !10x MOPS | Final Concentration | For 500 mL | For 1 Liter |
| 0.4 M Morpholinopropanesulfonic (MOPS) acid (free acid) | 41.9 g MOPS | 83.8 g MOPS | |
| 0.1 M Na-acetate-3 x H2O | 6.8 g | 13.6 g | |
| 10 mM EDTA | 10 mL 0.5 M EDTA | 20 mL 0.5 M EDTA | |
| Adjust pH to 7.2 with NaOH | |||
| Store in the Dark at 4 °C | |||
