Yu:Northern solns

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Northern Solutions

20x SSC
Final Concentration For 1 Liter For 4 Liters
3M NaCl 175.3 g 701.2 g
0.3M NaCitrate 88.2 g 352.8 g
milliQ H2O to 1 L to 4 L


10x MOPS
Final Concentration For 500 mL For 1 Liter
0.4 M Morpholinopropanesulfonic (MOPS) acid (free acid) 41.9 g MOPS 83.8 g MOPS
0.1 M Na-acetate-3 x H2O 6.8 g 13.6 g
10 mM EDTA 10 mL 0.5 M EDTA 20 mL 0.5 M EDTA
Adjust pH to 7.2 with NaOH
Store in the Dark at 4 °C