Yu:PBS

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m (New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1L 10x Phosphate-Buffered Saline. Dilute to 1x for use.''' |width="80%" valign="top"|1. Measure 700mL millipore wat...)
m (PBST)
Line 2: Line 2:
{| border="0" cellpadding="10"
{| border="0" cellpadding="10"
-
|+'''Solution recipe for 1L 10x Phosphate-Buffered Saline. Dilute to 1x for use.'''
+
|+'''Solution recipe for 1L 10x <span id="PBS">Phosphate-Buffered Saline</span>. Dilute to 1x for use.'''
-
|width="80%" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
+
|width="600px" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
-
|width="20%" valign="top"|700mL H<sub>2</sub>O
+
|width="100px" valign="top"|700mL H<sub>2</sub>O
|-
|-
|2. Weigh 80g NaCl and add it to the mixture
|2. Weigh 80g NaCl and add it to the mixture
|80g NaCl
|80g NaCl
|-
|-
-
|3. Weigh 2g KCl and add it to the mixture
+
|5. Weigh 2g KCl and add it to the mixture
|2g KCl
|2g KCl
|-
|-
Line 25: Line 25:
|-
|-
|8. Dilute from 10x to 1x (1:10) for use
|8. Dilute from 10x to 1x (1:10) for use
 +
|}
 +
 +
 +
{| border="0" cellpadding="10"
 +
|+'''Solution recipe for 1L 1x <span id="PBST">PBST</span>'''.
 +
|width="600px" valign="top"|1. Measure 1L 1x PBS into a 1L bottle.
 +
|width="100px" valign="top"|1L 1x [[Yu:PBS#PBS|PBS]]
 +
|-
 +
|2. Pipette 2.5mL TWEEN 20 into the bottle
 +
|2.5mL TWEEN 20
 +
|-
 +
|3. Stir until mixed
 +
|
|}
|}

Revision as of 13:19, 7 June 2009

Home       Procedures       | Lab Members       | Publications       | Research Interests       | Internal Database       Image:UBLogo.jpg


Solution recipe for 1L 10x Phosphate-Buffered Saline. Dilute to 1x for use.
1. Measure 700mL millipore water into a 2L plastic beaker. 700mL H2O
2. Weigh 80g NaCl and add it to the mixture 80g NaCl
5. Weigh 2g KCl and add it to the mixture 2g KCl
4. Weigh 14.4g Na2HPO4 and add it to the mixture 14.4g Na2HPO4
5. Weigh 2.4g KH2PO4 and add it to the mixture 2.4g KH2PO4
6. Stir until dissolved
7. Bring volume to 1L with millipore water in a graduated cylinder Volume to 1L
8. Dilute from 10x to 1x (1:10) for use


Solution recipe for 1L 1x PBST.
1. Measure 1L 1x PBS into a 1L bottle. 1L 1x PBS
2. Pipette 2.5mL TWEEN 20 into the bottle 2.5mL TWEEN 20
3. Stir until mixed
Personal tools