# Yu:PBS

(Difference between revisions)
 Revision as of 13:19, 7 June 2009 (view source)m (PBST)← Previous diff Revision as of 15:43, 7 June 2009 (view source)m (PBSMT)Next diff → Line 33: Line 33: |width="100px" valign="top"|1L 1x [[Yu:PBS#PBS|PBS]] |width="100px" valign="top"|1L 1x [[Yu:PBS#PBS|PBS]] |- |- - |2. Pipette 2.5mL TWEEN 20 into the bottle + |2. Pipette 2.5mL [[Tween|TWEEN 20]] into the bottle - |2.5mL TWEEN 20 + |2.5mL [[Tween|TWEEN 20]] |- |- |3. Stir until mixed |3. Stir until mixed | | + |} + + + {| border="0" cellpadding="10" + |+'''Solution recipe for 1L 1x PBSMT'''. + |width="600px" valign="top"|1. Measure 1L 1x PBS into a 1L bottle. + |width="100px" valign="top"|1L 1x [[Yu:PBS#PBS|PBS]] + |- + |2. Pipette 3mL 1M KCl into the bottle, to bring KCl concentration to 3mM + |3mL 1M KCl + |- + |3. Pipette 2.5mL 1M MgCl2 into the bottle, to bring MgCl2 concentration to 2.5mM + |2.5mL 1M MgCl2 + |- + |4. Pipette 5mL [[Triton X-100]] into the bottle, to bring Triton X-100 concentration to .5% + |5mL [[Triton X-100]] |} |}

## Revision as of 15:43, 7 June 2009

 1. Measure 700mL millipore water into a 2L plastic beaker. 700mL H2O 2. Weigh 80g NaCl and add it to the mixture 80g NaCl 5. Weigh 2g KCl and add it to the mixture 2g KCl 4. Weigh 14.4g Na2HPO4 and add it to the mixture 14.4g Na2HPO4 5. Weigh 2.4g KH2PO4 and add it to the mixture 2.4g KH2PO4 6. Stir until dissolved 7. Bring volume to 1L with millipore water in a graduated cylinder Volume to 1L 8. Dilute from 10x to 1x (1:10) for use

 1. Measure 1L 1x PBS into a 1L bottle. 1L 1x PBS 2. Pipette 2.5mL TWEEN 20 into the bottle 2.5mL TWEEN 20 3. Stir until mixed

 1. Measure 1L 1x PBS into a 1L bottle. 1L 1x PBS 2. Pipette 3mL 1M KCl into the bottle, to bring KCl concentration to 3mM 3mL 1M KCl 3. Pipette 2.5mL 1M MgCl2 into the bottle, to bring MgCl2 concentration to 2.5mM 2.5mL 1M MgCl2 4. Pipette 5mL Triton X-100 into the bottle, to bring Triton X-100 concentration to .5% 5mL Triton X-100