# Yu:PBS

(Difference between revisions)
 Revision as of 16:56, 22 September 2009 (view source)m ← Previous diff Revision as of 16:59, 22 September 2009 (view source)Next diff → Line 55: Line 55: |5mL [[Triton X-100]] |5mL [[Triton X-100]] |} |} + + {| border="0" cellpadding="10" + |+'''Solution recipe for PBS KMT '''. + |1. 20mL PBS (10X) + |- + |2. 600 μL 1M KCl=3mM Final concentration + |- + |3. 500μL 1M MgCl2=2.5mM final concentration + |- + |4. 10mL 10%-Triton X-100 + |- + |5. Fill to 200mL with Milipore H2O + |- + [[Category:Yu Solutions]] [[Category:Yu Solutions]]

## Revision as of 16:59, 22 September 2009

 1. Measure 700mL millipore water into a 2L plastic beaker. 700mL H2O 2. Weigh 80g NaCl and add it to the mixture 80g NaCl 5. Weigh 2g KCl and add it to the mixture 2g KCl 4. Weigh 14.4g Na2HPO4 and add it to the mixture 14.4g Na2HPO4 5. Weigh 2.4g KH2PO4 and add it to the mixture 2.4g KH2PO4 6. Stir until dissolved 7. Bring volume to 1L with millipore water in a graduated cylinder Volume to 1L 8. Dilute from 10x to 1x (1:10) for use

 1. Measure 1L 1x PBS into a 1L bottle. 1L 1x PBS 2. Pipette 2.5mL TWEEN 20 into the bottle 2.5mL TWEEN 20 3. Stir until mixed

 1. Measure 1L 1x PBS into a 1L bottle. 1L 1x PBS 2. Pipette 3mL 1M KCl into the bottle, to bring KCl concentration to 3mM 3mL 1M KCl 3. Pipette 2.5mL 1M MgCl2 into the bottle, to bring MgCl2 concentration to 2.5mM 2.5mL 1M MgCl2 4. Pipette 5mL Triton X-100 into the bottle, to bring Triton X-100 concentration to .5% 5mL Triton X-100
 1. 20mL PBS (10X) 2. 600 μL 1M KCl=3mM Final concentration 3. 500μL 1M MgCl2=2.5mM final concentration 4. 10mL 10%-Triton X-100 5. Fill to 200mL with Milipore H2O