Yu:PBS

(Difference between revisions)

Revision as of 16:59, 22 September 2009

Home Procedures | Lab Members | Publications | Research Interests | Internal Database

**Solution recipe for 1L 10x Phosphate-Buffered Saline. Dilute to 1x for use.**
1. Measure 700mL millipore water into a 2L plastic beaker.	700mL H₂O
2. Weigh 80g NaCl and add it to the mixture	80g NaCl
5. Weigh 2g KCl and add it to the mixture	2g KCl
4. Weigh 14.4g Na₂HPO₄ and add it to the mixture	14.4g Na₂HPO₄
5. Weigh 2.4g KH₂PO₄ and add it to the mixture	2.4g KH₂PO₄
6. Stir until dissolved
7. Bring volume to 1L with millipore water in a graduated cylinder	Volume to 1L
8. Dilute from 10x to 1x (1:10) for use

**Solution recipe for 1L 1x PBST**.
1. Measure 1L 1x PBS into a 1L bottle.	1L 1x PBS
2. Pipette 2.5mL TWEEN 20 into the bottle	2.5mL TWEEN 20
3. Stir until mixed

**Solution recipe for 1L 1x PBSMT**.
1. Measure 1L 1x PBS into a 1L bottle.	1L 1x PBS
2. Pipette 3mL 1M KCl into the bottle, to bring KCl concentration to 3mM	3mL 1M KCl
3. Pipette 2.5mL 1M MgCl₂ into the bottle, to bring MgCl₂ concentration to 2.5mM	2.5mL 1M MgCl₂
4. Pipette 5mL Triton X-100 into the bottle, to bring Triton X-100 concentration to .5%	5mL Triton X-100

**Solution recipe for PBS KMT** .
1. 20mL PBS (10X)
2. 600 μL 1M KCl=3mM Final concentration
3. 500μL 1M MgCl₂=2.5mM final concentration
4. 10mL 10%-Triton X-100
5. Fill to 200mL with Milipore H₂O

@@ Line 55: / Line 55: @@
 |5mL [[Triton X-100]]
 |}
+{| border="0" cellpadding="10"
+|+'''Solution recipe for PBS KMT '''.
+|1. 20mL PBS (10X)
+|-
+|2. 600 μL 1M KCl=3mM Final concentration
+|-
+|3. 500μL 1M MgCl<sub>2</sub>=2.5mM final concentration
+|-
+|4. 10mL 10%-Triton X-100
+|-
+|5. Fill to 200mL with Milipore H<sub>2</sub>O
+|-
 [[Category:Yu Solutions]]