Yu:PBS - Revision history

Christopher Jackson: fix

2010-05-14T22:41:04Z

fix

←Older revision		Revision as of 22:41, 14 May 2010
Line 43:		Line 43:
	{\| border="0" cellpadding="10"		{\| border="0" cellpadding="10"
	\|+'''Solution recipe for 1L 1x <span id="PBSMT">PBSMT</span>'''.		\|+'''Solution recipe for 1L 1x <span id="PBSMT">PBSMT</span>'''.
-	\|width="600px" valign="top"\|1. Measure ~~1L 1x~~ PBS into a ~~1L bottle~~.	+	\|width="600px" valign="top"\|1. Measure 100mL 10x PBS into a beaker.
-	\|width="100px" valign="top"\|1L 1x [[Yu:PBS#PBS\|PBS]]	+	\|width="100px" valign="top"\|1x [[Yu:PBS#PBS\|PBS]]
	\|-		\|-
	\|2. Add 3mL 1M KCl		\|2. Add 3mL 1M KCl
Line 55:		Line 55:
	\|0.5% [[Triton X-100]]		\|0.5% [[Triton X-100]]
	\|-		\|-
-	\|5. ~~Stir~~ until mixed	+	\|5. Bring to 1L volume with H<sub>2</sub>O and stir until mixed
	\|		\|
	\|}		\|}

	[[Category:Yu Solutions]]		[[Category:Yu Solutions]]

Christopher Jackson: fixed mMs

2010-05-09T23:53:21Z

fixed mMs

←Older revision		Revision as of 23:53, 9 May 2010
Line 4:		Line 4:
	\|+'''Solution recipe for 1L 10x <span id="PBS">Phosphate-Buffered Saline</span>. Dilute to 1x for use.'''		\|+'''Solution recipe for 1L 10x <span id="PBS">Phosphate-Buffered Saline</span>. Dilute to 1x for use.'''
	\|width="600px" valign="top"\|1. Measure 700mL millipore water into a 2L plastic beaker.		\|width="600px" valign="top"\|1. Measure 700mL millipore water into a 2L plastic beaker.
-	\|width="100px" valign="top"\|~~700mL H~~<~~sub~~>2</~~sub~~>O	+	\|width="100px" valign="top"\|<u>10x</u>
	\|-		\|-
	\|2. Weigh 80g NaCl and add it to the mixture		\|2. Weigh 80g NaCl and add it to the mixture
-	\|~~80g~~ NaCl	+	\|1.4M NaCl
	\|-		\|-
	\|5. Weigh 2g KCl and add it to the mixture		\|5. Weigh 2g KCl and add it to the mixture
-	\|2g KCl	+	\|30mM KCl
	\|-		\|-
	\|4. Weigh 14.4g Na<sub>2</sub>HPO<sub>4</sub> and add it to the mixture		\|4. Weigh 14.4g Na<sub>2</sub>HPO<sub>4</sub> and add it to the mixture
-	\|~~14.4g~~ Na<sub>2</sub>HPO<sub>4</sub>	+	\|100mM Na<sub>2</sub>HPO<sub>4</sub>
	\|-		\|-
	\|5. Weigh 2.4g KH<sub>2</sub>PO<sub>4</sub> and add it to the mixture		\|5. Weigh 2.4g KH<sub>2</sub>PO<sub>4</sub> and add it to the mixture
-	\|~~2.4g~~ KH<sub>2</sub>PO<sub>4</sub>	+	\|20mM KH<sub>2</sub>PO<sub>4</sub>
	\|-		\|-
	\|6. Stir until dissolved		\|6. Stir until dissolved
Line 22:		Line 22:
	\|-		\|-
	\|7. Bring volume to 1L with millipore water in a graduated cylinder		\|7. Bring volume to 1L with millipore water in a graduated cylinder
-	\|~~Volume to 1L~~	+	\|
	\|-		\|-
	\|8. Dilute from 10x to 1x (1:10) for use		\|8. Dilute from 10x to 1x (1:10) for use
Line 34:		Line 34:
	\|-		\|-
	\|2. Pipette 2.5mL [[Tween\|TWEEN 20]] into the bottle		\|2. Pipette 2.5mL [[Tween\|TWEEN 20]] into the bottle
-	\|2.~~5mL~~ [[Tween\|TWEEN 20]]	+	\|0.25% [[Tween\|TWEEN 20]]
	\|-		\|-
	\|3. Stir until mixed		\|3. Stir until mixed
Line 46:		Line 46:
	\|width="100px" valign="top"\|1L 1x [[Yu:PBS#PBS\|PBS]]		\|width="100px" valign="top"\|1L 1x [[Yu:PBS#PBS\|PBS]]
	\|-		\|-
-	\|2. ~~Pipette~~ 3mL 1M KCl ~~into the bottle, to bring KCl concentration to 3mM~~	+	\|2. Add 3mL 1M KCl
-	\|~~3mL 1M~~ KCl	+	\|3mM KCl
	\|-		\|-
-	\|3. ~~Pipette~~ 2.5mL 1M MgCl<sub>2</sub> ~~into the bottle, to bring MgCl<sub>2</sub> concentration to 2.5mM~~	+	\|3. Add 2.5mL 1M MgCl<sub>2</sub>
-	\|2.~~5mL 1M~~ MgCl<sub>2</sub>	+	\|2.5mM MgCl<sub>2</sub>
	\|-		\|-
-	\|4. ~~Pipette~~ 5mL [[Triton X-100]] ~~into the bottle, to bring Triton X-100 concentration to~~ .5%	+	\|4. Add 5mL [[Triton X-100]]
-	~~\|5mL~~ [[Triton X-100]]	+	\|0.5% [[Triton X-100]]
		+	\|-
		+	\|5. Stir until mixed
		+	\|
	\|}		\|}

	[[Category:Yu Solutions]]		[[Category:Yu Solutions]]

Eric Milliman at 21:01, 22 September 2009

2009-09-22T21:01:18Z

←Older revision		Revision as of 21:01, 22 September 2009
Line 55:		Line 55:
	\|5mL [[Triton X-100]]		\|5mL [[Triton X-100]]
	\|}		\|}
-
-	~~{\| border="0" cellpadding="10"~~
-	~~\|+'''Solution recipe for PBS KMT '''.~~
-	~~\|1. 20mL PBS (10X)~~
-	\|-
-	~~\|2. 600 μL 1M KCl=3mM Final concentration~~
-	\|-
-	~~\|3. 500μL 1M MgCl<sub>2</sub>=2.5mM final concentration~~
-	\|-
-	~~\|4. 10mL 10%-Triton X-100~~
-	\|-
-	~~\|5. Fill to 200mL with Milipore H<sub>2</sub>O~~
-	\|-
-

	[[Category:Yu Solutions]]		[[Category:Yu Solutions]]

Eric Milliman at 20:59, 22 September 2009

2009-09-22T20:59:26Z

←Older revision		Revision as of 20:59, 22 September 2009
Line 55:		Line 55:
	\|5mL [[Triton X-100]]		\|5mL [[Triton X-100]]
	\|}		\|}
		+
		+	{\| border="0" cellpadding="10"
		+	\|+'''Solution recipe for PBS KMT '''.
		+	\|1. 20mL PBS (10X)
		+	\|-
		+	\|2. 600 μL 1M KCl=3mM Final concentration
		+	\|-
		+	\|3. 500μL 1M MgCl<sub>2</sub>=2.5mM final concentration
		+	\|-
		+	\|4. 10mL 10%-Triton X-100
		+	\|-
		+	\|5. Fill to 200mL with Milipore H<sub>2</sub>O
		+	\|-
		+

	[[Category:Yu Solutions]]		[[Category:Yu Solutions]]

Christopher Jackson at 20:56, 22 September 2009

2009-09-22T20:56:03Z

←Older revision		Revision as of 20:56, 22 September 2009
Line 55:		Line 55:
	\|5mL [[Triton X-100]]		\|5mL [[Triton X-100]]
	\|}		\|}
		+
		+	[[Category:Yu Solutions]]

Michaelcyu: PBSMT

2009-06-07T19:43:35Z

PBSMT

←Older revision		Revision as of 19:43, 7 June 2009
Line 33:		Line 33:
	\|width="100px" valign="top"\|1L 1x [[Yu:PBS#PBS\|PBS]]		\|width="100px" valign="top"\|1L 1x [[Yu:PBS#PBS\|PBS]]
	\|-		\|-
-	\|2. Pipette 2.5mL TWEEN 20 into the bottle	+	\|2. Pipette 2.5mL [[Tween\|TWEEN 20]] into the bottle
-	\|2.5mL TWEEN 20	+	\|2.5mL [[Tween\|TWEEN 20]]
	\|-		\|-
	\|3. Stir until mixed		\|3. Stir until mixed
	\|		\|
		+	\|}
		+
		+
		+	{\| border="0" cellpadding="10"
		+	\|+'''Solution recipe for 1L 1x <span id="PBSMT">PBSMT</span>'''.
		+	\|width="600px" valign="top"\|1. Measure 1L 1x PBS into a 1L bottle.
		+	\|width="100px" valign="top"\|1L 1x [[Yu:PBS#PBS\|PBS]]
		+	\|-
		+	\|2. Pipette 3mL 1M KCl into the bottle, to bring KCl concentration to 3mM
		+	\|3mL 1M KCl
		+	\|-
		+	\|3. Pipette 2.5mL 1M MgCl<sub>2</sub> into the bottle, to bring MgCl<sub>2</sub> concentration to 2.5mM
		+	\|2.5mL 1M MgCl<sub>2</sub>
		+	\|-
		+	\|4. Pipette 5mL [[Triton X-100]] into the bottle, to bring Triton X-100 concentration to .5%
		+	\|5mL [[Triton X-100]]
	\|}		\|}

Michaelcyu: PBST

2009-06-07T17:19:07Z

PBST

←Older revision		Revision as of 17:19, 7 June 2009
Line 2:		Line 2:

	{\| border="0" cellpadding="10"		{\| border="0" cellpadding="10"
-	\|+'''Solution recipe for 1L 10x Phosphate-Buffered Saline. Dilute to 1x for use.'''	+	\|+'''Solution recipe for 1L 10x <span id="PBS">Phosphate-Buffered Saline</span>. Dilute to 1x for use.'''
-	\|width="~~80%~~" valign="top"\|1. Measure 700mL millipore water into a 2L plastic beaker.	+	\|width="600px" valign="top"\|1. Measure 700mL millipore water into a 2L plastic beaker.
-	\|width="~~20%~~" valign="top"\|700mL H<sub>2</sub>O	+	\|width="100px" valign="top"\|700mL H<sub>2</sub>O
	\|-		\|-
	\|2. Weigh 80g NaCl and add it to the mixture		\|2. Weigh 80g NaCl and add it to the mixture
	\|80g NaCl		\|80g NaCl
	\|-		\|-
-	\|3. Weigh 2g KCl and add it to the mixture	+	\|5. Weigh 2g KCl and add it to the mixture
	\|2g KCl		\|2g KCl
	\|-		\|-
Line 25:		Line 25:
	\|-		\|-
	\|8. Dilute from 10x to 1x (1:10) for use		\|8. Dilute from 10x to 1x (1:10) for use
		+	\|}
		+
		+
		+	{\| border="0" cellpadding="10"
		+	\|+'''Solution recipe for 1L 1x <span id="PBST">PBST</span>'''.
		+	\|width="600px" valign="top"\|1. Measure 1L 1x PBS into a 1L bottle.
		+	\|width="100px" valign="top"\|1L 1x [[Yu:PBS#PBS\|PBS]]
		+	\|-
		+	\|2. Pipette 2.5mL TWEEN 20 into the bottle
		+	\|2.5mL TWEEN 20
		+	\|-
		+	\|3. Stir until mixed
		+	\|
	\|}		\|}

Christopher Jackson: New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1L 10x Phosphate-Buffered Saline. Dilute to 1x for use.''' |width="80%" valign="top"|1. Measure 700mL millipore wat...

2009-06-06T21:36:37Z

New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1L 10x Phosphate-Buffered Saline. Dilute to 1x for use.''' |width="80%" valign="top"|1. Measure 700mL millipore wat...

New page

{{Template:Yu}}

{| border="0" cellpadding="10"
|+'''Solution recipe for 1L 10x Phosphate-Buffered Saline. Dilute to 1x for use.'''
|width="80%" valign="top"|1. Measure 700mL millipore water into a 2L plastic beaker.
|width="20%" valign="top"|700mL H2O
|-
|2. Weigh 80g NaCl and add it to the mixture
|80g NaCl
|-
|3. Weigh 2g KCl and add it to the mixture
|2g KCl
|-
|4. Weigh 14.4g Na2HPO4 and add it to the mixture
|14.4g Na2HPO4
|-
|5. Weigh 2.4g KH2PO4 and add it to the mixture
|2.4g KH2PO4
|-
|6. Stir until dissolved
|
|-
|7. Bring volume to 1L with millipore water in a graduated cylinder
|Volume to 1L
|-
|8. Dilute from 10x to 1x (1:10) for use
|}