Yu:PBS
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1. Measure 700mL millipore water into a 2L plastic beaker. | 700mL H2O |
2. Weigh 80g NaCl and add it to the mixture | 80g NaCl |
5. Weigh 2g KCl and add it to the mixture | 2g KCl |
4. Weigh 14.4g Na2HPO4 and add it to the mixture | 14.4g Na2HPO4 |
5. Weigh 2.4g KH2PO4 and add it to the mixture | 2.4g KH2PO4 |
6. Stir until dissolved | |
7. Bring volume to 1L with millipore water in a graduated cylinder | Volume to 1L |
8. Dilute from 10x to 1x (1:10) for use |
1. Measure 1L 1x PBS into a 1L bottle. | 1L 1x PBS |
2. Pipette 2.5mL TWEEN 20 into the bottle | 2.5mL TWEEN 20 |
3. Stir until mixed |