Yu:PBS
From OpenWetWare
1. Measure 700mL millipore water into a 2L plastic beaker. | 700mL H2O |
2. Weigh 80g NaCl and add it to the mixture | 80g NaCl |
5. Weigh 2g KCl and add it to the mixture | 2g KCl |
4. Weigh 14.4g Na2HPO4 and add it to the mixture | 14.4g Na2HPO4 |
5. Weigh 2.4g KH2PO4 and add it to the mixture | 2.4g KH2PO4 |
6. Stir until dissolved | |
7. Bring volume to 1L with millipore water in a graduated cylinder | Volume to 1L |
8. Dilute from 10x to 1x (1:10) for use |
1. Measure 1L 1x PBS into a 1L bottle. | 1L 1x PBS |
2. Pipette 2.5mL TWEEN 20 into the bottle | 2.5mL TWEEN 20 |
3. Stir until mixed |
1. Measure 1L 1x PBS into a 1L bottle. | 1L 1x PBS |
2. Pipette 3mL 1M KCl into the bottle, to bring KCl concentration to 3mM | 3mL 1M KCl |
3. Pipette 2.5mL 1M MgCl2 into the bottle, to bring MgCl2 concentration to 2.5mM | 2.5mL 1M MgCl2 |
4. Pipette 5mL Triton X-100 into the bottle, to bring Triton X-100 concentration to .5% | 5mL Triton X-100 |