Yu:PLAAC

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PLAAC is a standard protease inhibitor cocktail for yeast work. Stock concentration is 1000X (add 1μL per 1mL of lysis buffer). Thaw at room temperature due to DMSO. Keep PLAAC-complete solutions chilled.
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{| border="0" cellpadding="10"
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|+'''Solution recipe for PLAAC(Protease inhibitor cocktail for yeast)'''.
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|+'''Solution recipe for PLAAC Stock'''.
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| 1:1:1:1 of lab stock=1000x
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| 3ng/μL Pepstatin
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| Can add PMSF(1mM final)
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| 3ng/μL Leupeptin
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| Final concentration =3ng/μL of Pepstatin, Leu, Apro, Chy, Antipain
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| 3ng/μL Antipain
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|-
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| 3ng/μL Aprotinin
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| 3ng/μL Chymostatin
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|Dissolve in DMSO. Aliquot 100μL per tube and freeze at -20°C.
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|}
[[Category:Yu Solutions]]
[[Category:Yu Solutions]]

Current revision

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PLAAC is a standard protease inhibitor cocktail for yeast work. Stock concentration is 1000X (add 1μL per 1mL of lysis buffer). Thaw at room temperature due to DMSO. Keep PLAAC-complete solutions chilled.

Solution recipe for PLAAC Stock.
3ng/μL Pepstatin
3ng/μL Leupeptin
3ng/μL Antipain
3ng/μL Aprotinin
3ng/μL Chymostatin
Dissolve in DMSO. Aliquot 100μL per tube and freeze at -20°C.
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